Effects of Host Plant Factors on the Bacterial Communities Associated with Two Whitefly Sibling Species.

BACKGROUND:Although discrepancy in the specific traits and ecological characteristics of Bemisia tabaci between species are partially attributed to the B. tabaci-associated bacteria, the factors that affect the diversity of B. tabaci-associated bacteria are not well-understood. We used the metagenom...

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Main Authors: Ming-Ming Su, Lei Guo, Yun-Li Tao, You-Jun Zhang, Fang-Hao Wan, Dong Chu
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2016-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4805303?pdf=render
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spelling doaj-9a79e0a144b448dead155e08e72e54452020-11-25T00:05:47ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01113e015218310.1371/journal.pone.0152183Effects of Host Plant Factors on the Bacterial Communities Associated with Two Whitefly Sibling Species.Ming-Ming SuLei GuoYun-Li TaoYou-Jun ZhangFang-Hao WanDong ChuBACKGROUND:Although discrepancy in the specific traits and ecological characteristics of Bemisia tabaci between species are partially attributed to the B. tabaci-associated bacteria, the factors that affect the diversity of B. tabaci-associated bacteria are not well-understood. We used the metagenomic approach to characterize the B. tabaci-associated bacterial community because the approach is an effective tool to identify the bacteria. METHODOLOGY AND RESULTS:To investigate the effects of the host plant and a virus, tomato yellow leaf curl virus (TYLCV), on the bacterial communities of B. tabaci sibling species B and Q, we analyzed the bacterial communities associated with whitefly B and Q collected from healthy cotton, healthy tomato, and TYLCV-infected tomato. The analysis used miseq-based sequencing of a variable region of the bacterial 16S rDNA gene. For the bacteria associated with B. tabaci, we found that the influence of the host plant species was greater than that of the whitefly cryptic species. With further analysis of host plants infected with the TYLCV, the virus had no significant effects on the B. tabaci-associated bacterial community. CONCLUSIONS:The effects of different plant hosts and TYLCV-infection on the diversity of B. tabaci-associated bacterial communities were successfully analyzed in this study. To explain why B. tabaci sibling species with different host ranges differ in performance, the analysis of the bacterial community may be essential to the explanation.http://europepmc.org/articles/PMC4805303?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Ming-Ming Su
Lei Guo
Yun-Li Tao
You-Jun Zhang
Fang-Hao Wan
Dong Chu
spellingShingle Ming-Ming Su
Lei Guo
Yun-Li Tao
You-Jun Zhang
Fang-Hao Wan
Dong Chu
Effects of Host Plant Factors on the Bacterial Communities Associated with Two Whitefly Sibling Species.
PLoS ONE
author_facet Ming-Ming Su
Lei Guo
Yun-Li Tao
You-Jun Zhang
Fang-Hao Wan
Dong Chu
author_sort Ming-Ming Su
title Effects of Host Plant Factors on the Bacterial Communities Associated with Two Whitefly Sibling Species.
title_short Effects of Host Plant Factors on the Bacterial Communities Associated with Two Whitefly Sibling Species.
title_full Effects of Host Plant Factors on the Bacterial Communities Associated with Two Whitefly Sibling Species.
title_fullStr Effects of Host Plant Factors on the Bacterial Communities Associated with Two Whitefly Sibling Species.
title_full_unstemmed Effects of Host Plant Factors on the Bacterial Communities Associated with Two Whitefly Sibling Species.
title_sort effects of host plant factors on the bacterial communities associated with two whitefly sibling species.
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2016-01-01
description BACKGROUND:Although discrepancy in the specific traits and ecological characteristics of Bemisia tabaci between species are partially attributed to the B. tabaci-associated bacteria, the factors that affect the diversity of B. tabaci-associated bacteria are not well-understood. We used the metagenomic approach to characterize the B. tabaci-associated bacterial community because the approach is an effective tool to identify the bacteria. METHODOLOGY AND RESULTS:To investigate the effects of the host plant and a virus, tomato yellow leaf curl virus (TYLCV), on the bacterial communities of B. tabaci sibling species B and Q, we analyzed the bacterial communities associated with whitefly B and Q collected from healthy cotton, healthy tomato, and TYLCV-infected tomato. The analysis used miseq-based sequencing of a variable region of the bacterial 16S rDNA gene. For the bacteria associated with B. tabaci, we found that the influence of the host plant species was greater than that of the whitefly cryptic species. With further analysis of host plants infected with the TYLCV, the virus had no significant effects on the B. tabaci-associated bacterial community. CONCLUSIONS:The effects of different plant hosts and TYLCV-infection on the diversity of B. tabaci-associated bacterial communities were successfully analyzed in this study. To explain why B. tabaci sibling species with different host ranges differ in performance, the analysis of the bacterial community may be essential to the explanation.
url http://europepmc.org/articles/PMC4805303?pdf=render
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