Multivariate analysis as a tool for measuring the stability of morphometric traits in Lycopersicon plants from in vitro culture

The phenotypic stability of morphometric traits in Lycopersicon spp. (stem perimeter at the base, middle and top, and number of flowers per cluster) was measured by multivariate analysis through a progeny test in order to estimate the genetic stability of these traits. Principal components were calc...

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Main Authors: Guillermo Pratta, Roxana Zorzoli, Liliana Amelia Picardi
Format: Article
Language:English
Published: Sociedade Brasileira de Genética 2000-06-01
Series:Genetics and Molecular Biology
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200039
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spelling doaj-9a73d5eba1d44641b94a4c2d52559e7b2020-11-25T02:51:17ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852000-06-0123247949310.1590/S1415-47572000000200039Multivariate analysis as a tool for measuring the stability of morphometric traits in Lycopersicon plants from in vitro cultureGuillermo PrattaRoxana ZorzoliLiliana Amelia PicardiThe phenotypic stability of morphometric traits in Lycopersicon spp. (stem perimeter at the base, middle and top, and number of flowers per cluster) was measured by multivariate analysis through a progeny test in order to estimate the genetic stability of these traits. Principal components were calculated for two groups of Lycopersicon spp., non-regenerated plants and the progeny of regenerated plants. Analysis of variance was performed to support principal component analysis. Both groups presented similar eigenvalues and eigenvectors, while no significant differences were found between any of the traits studied. These results indicated that the phenotypic structure was the same among the progeny of regenerated and non-regenerated plants, so that no variation would occur in in vitro culture. Multivariate analysis proved to be an appropriate methodology for the measurement of the stability of morphometric traits after one regeneration cycle.<br>Nesta experiência a estabilidade fenotípica dos caracteres métricos perímetro do culmo na base, no meio e no alto e número de flores por racemo foi medida mediante a prova de progênie com análise multivariada. Desta forma, uma nova metodologia para avaliação da estabilidade genética depois de um ciclo de regeneração in vitro foi proposta. Os componentes principais foram calculados para dois grupos de plantas de Lycopersicon spp.: o não regenerado e a progênie das plantas regeneradas. A ANOVA foi empregada como controle da análise de componentes principais. As diferenças entre grupos não foram estatisticamente significantes para nenhum caráter. Ambos os grupos apresentaram autovalores e autovetores similares. Os resultados obtidos indicaram que a estrutura fenotípica das plantas não foi modificada. Assim, nenhuma variação foi causada pela cultura in vitro. A análise multivariada mostrou ser uma metodologia apropriada para a medida da estabilidade dos caracteres métricos depois de um ciclo de regeneração.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200039
collection DOAJ
language English
format Article
sources DOAJ
author Guillermo Pratta
Roxana Zorzoli
Liliana Amelia Picardi
spellingShingle Guillermo Pratta
Roxana Zorzoli
Liliana Amelia Picardi
Multivariate analysis as a tool for measuring the stability of morphometric traits in Lycopersicon plants from in vitro culture
Genetics and Molecular Biology
author_facet Guillermo Pratta
Roxana Zorzoli
Liliana Amelia Picardi
author_sort Guillermo Pratta
title Multivariate analysis as a tool for measuring the stability of morphometric traits in Lycopersicon plants from in vitro culture
title_short Multivariate analysis as a tool for measuring the stability of morphometric traits in Lycopersicon plants from in vitro culture
title_full Multivariate analysis as a tool for measuring the stability of morphometric traits in Lycopersicon plants from in vitro culture
title_fullStr Multivariate analysis as a tool for measuring the stability of morphometric traits in Lycopersicon plants from in vitro culture
title_full_unstemmed Multivariate analysis as a tool for measuring the stability of morphometric traits in Lycopersicon plants from in vitro culture
title_sort multivariate analysis as a tool for measuring the stability of morphometric traits in lycopersicon plants from in vitro culture
publisher Sociedade Brasileira de Genética
series Genetics and Molecular Biology
issn 1415-4757
1678-4685
publishDate 2000-06-01
description The phenotypic stability of morphometric traits in Lycopersicon spp. (stem perimeter at the base, middle and top, and number of flowers per cluster) was measured by multivariate analysis through a progeny test in order to estimate the genetic stability of these traits. Principal components were calculated for two groups of Lycopersicon spp., non-regenerated plants and the progeny of regenerated plants. Analysis of variance was performed to support principal component analysis. Both groups presented similar eigenvalues and eigenvectors, while no significant differences were found between any of the traits studied. These results indicated that the phenotypic structure was the same among the progeny of regenerated and non-regenerated plants, so that no variation would occur in in vitro culture. Multivariate analysis proved to be an appropriate methodology for the measurement of the stability of morphometric traits after one regeneration cycle.<br>Nesta experiência a estabilidade fenotípica dos caracteres métricos perímetro do culmo na base, no meio e no alto e número de flores por racemo foi medida mediante a prova de progênie com análise multivariada. Desta forma, uma nova metodologia para avaliação da estabilidade genética depois de um ciclo de regeneração in vitro foi proposta. Os componentes principais foram calculados para dois grupos de plantas de Lycopersicon spp.: o não regenerado e a progênie das plantas regeneradas. A ANOVA foi empregada como controle da análise de componentes principais. As diferenças entre grupos não foram estatisticamente significantes para nenhum caráter. Ambos os grupos apresentaram autovalores e autovetores similares. Os resultados obtidos indicaram que a estrutura fenotípica das plantas não foi modificada. Assim, nenhuma variação foi causada pela cultura in vitro. A análise multivariada mostrou ser uma metodologia apropriada para a medida da estabilidade dos caracteres métricos depois de um ciclo de regeneração.
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572000000200039
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