Enucleation of Buffalo Oocytes: A Comparison of Methods

Enucleation of Buffalo Oocytes: A Comparison of Methods

A comparative study of three enucleation methods; enucleation by pushing out small amount of cytoplasm beneath the first polar body, enucleation by bisectioning of oocytes, and enucleation by aspiration were carried out using the oocytes of Indian buffaloes. The statistical analysis of the results r...

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Main Authors: Lakshman Sahoo, Suresh K. Singla
Format: Article
Language:English
Published: Assiut University 2013-01-01
Series:Journal of Advanced Veterinary Research
Online Access:http://advetresearch.com/index.php/AVR/article/view/106
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spelling doaj-9a3fcc3f8897467b92ebd35fca18c3b92020-11-24T23:03:22ZengAssiut UniversityJournal of Advanced Veterinary Research2090-62692090-62772013-01-01311619106Enucleation of Buffalo Oocytes: A Comparison of MethodsLakshman SahooSuresh K. SinglaA comparative study of three enucleation methods; enucleation by pushing out small amount of cytoplasm beneath the first polar body, enucleation by bisectioning of oocytes, and enucleation by aspiration were carried out using the oocytes of Indian buffaloes. The statistical analysis of the results revealed that, there is no significant difference between the three enucleation methods. This information would be helpful for optimization of enucleation of recipient oocyte during somatic cell nuclear transfer.http://advetresearch.com/index.php/AVR/article/view/106
collection DOAJ
language English
format Article
sources DOAJ
author Lakshman Sahoo
Suresh K. Singla
spellingShingle Lakshman Sahoo
Suresh K. Singla
Enucleation of Buffalo Oocytes: A Comparison of Methods
Journal of Advanced Veterinary Research
author_facet Lakshman Sahoo
Suresh K. Singla
author_sort Lakshman Sahoo
title Enucleation of Buffalo Oocytes: A Comparison of Methods
title_short Enucleation of Buffalo Oocytes: A Comparison of Methods
title_full Enucleation of Buffalo Oocytes: A Comparison of Methods
title_fullStr Enucleation of Buffalo Oocytes: A Comparison of Methods
title_full_unstemmed Enucleation of Buffalo Oocytes: A Comparison of Methods
title_sort enucleation of buffalo oocytes: a comparison of methods
publisher Assiut University
series Journal of Advanced Veterinary Research
issn 2090-6269
2090-6277
publishDate 2013-01-01
description A comparative study of three enucleation methods; enucleation by pushing out small amount of cytoplasm beneath the first polar body, enucleation by bisectioning of oocytes, and enucleation by aspiration were carried out using the oocytes of Indian buffaloes. The statistical analysis of the results revealed that, there is no significant difference between the three enucleation methods. This information would be helpful for optimization of enucleation of recipient oocyte during somatic cell nuclear transfer.
url http://advetresearch.com/index.php/AVR/article/view/106
work_keys_str_mv AT lakshmansahoo enucleationofbuffalooocytesacomparisonofmethods
AT sureshksingla enucleationofbuffalooocytesacomparisonofmethods
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