Enucleation of Buffalo Oocytes: A Comparison of Methods
A comparative study of three enucleation methods; enucleation by pushing out small amount of cytoplasm beneath the first polar body, enucleation by bisectioning of oocytes, and enucleation by aspiration were carried out using the oocytes of Indian buffaloes. The statistical analysis of the results r...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Assiut University
2013-01-01
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| Series: | Journal of Advanced Veterinary Research |
| Online Access: | http://advetresearch.com/index.php/AVR/article/view/106 |
| Summary: | A comparative study of three enucleation methods; enucleation by pushing out small amount of cytoplasm beneath the first polar body, enucleation by bisectioning of oocytes, and enucleation by aspiration were carried out using the oocytes of Indian buffaloes. The statistical analysis of the results revealed that, there is no significant difference between the three enucleation methods. This information would be helpful for optimization of enucleation of recipient oocyte during somatic cell nuclear transfer. |
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| ISSN: | 2090-6269 2090-6277 |