The expression of miRNA⁃210⁃5P and bioinformatics analysis of its predicted target genes in peripheral blood of patients with carotid artherosclerotic stenosis

• Main Authors: Pan HUANG, Min XU, Xiao⁃ying HE
• Format: Article
• Language: English
• Published: Tianjin Huanhu Hospital 2019-07-01
• Series: Chinese Journal of Contemporary Neurology and Neurosurgery
• Subjects: Carotid stenosis; Atherosclerosis; MicroRNAs; Genes; Computational biology
• Online Access: http://www.cjcnn.org/index.php/cjcnn/article/view/1985
• ISSN: 1672-6731; 1672-6731

Objective To investigate the relative expression of miRNA⁃210⁃5P in serum of patients with carotid atherosclerotic stenosis (CAS) and to explore the function of miRNA⁃210⁃5P and its target genes using bioinformatics methods. Methods We selected 146 patients with CAS from July 2015 to September 2018 in our hospital. Reverse transcriptase⁃polymerase chain reaction (RT⁃PCR) was used to detect the relative expression of miRNA⁃210⁃5P in peripheral blood of all enrolled patients. The target genes were predicted by using TargetScan and CoMeTa databases. The target genes of miRNA⁃210⁃5P were enriched by Gene Ontology (GO) using DAVID data and were performed with KEGG Pathway analysis. Results Compared with non⁃CAS patients or normal subjects (control group, N = 60), the relative expression of miRNA⁃210⁃5P in the serum of CAS group was significantly increased(t=14.759, P=0.000). The relative expressions of serum miRNA⁃210⁃5P in severe stenosis group(N=31; q=23.028, P=0.000), moderate stenosis group (N=53; q=6.657, P=0.000) and mild stenosis group (N=62; q=42.612, P= 0.000) were higher than that in control group. The relative expressions of serum miRNA⁃210⁃5P in moderate stenosis group (q =34.538, P =0.000) and severe stenosis group (q =11.914, P =0.000) were significantly higher than that in mild stenosis group. There lative expressions of serum miRNA⁃210⁃5P in severe stenosis group was significantly higher than thatin moderate stenosis group (q=16.983, P=0.000). Receive roperating characteristic (ROC) curve showed that the miRNA⁃210⁃5P predicted the area under the curve (AUC) of moderate to severe stenosis in CAS to be 0.943, the sensitivity was 90.33% and the specificity was 92.54% at the best cutoff value of 1.495. Bioinformatics analysis showed there were 54 potential target genes of miRNA⁃210⁃5P, such as VEGFA, KCMF1, HMGCS1, KLF12, EFNA3, GIT2, etc. GO analysis showed that the target genes of miRNA⁃210⁃5P were involved in angiogenesis, neuronal development, positive regulation of DNA transcription factor activity, endothelial cell chemotaxis, cell migration and differentiation and adhesion processes. KEGG Pathway analysis displayed miRNA⁃210⁃5P target genes were mainly enriched in synaptic⁃directed factor signal transduction pathways. Conclusions The expression of miRNA⁃210⁃5P inperipheral blood of CAS patients is upregulated,and it may participate in the process of CAS occurrence and development by regulating multiple target genes and acting on synaptic⁃directed signaling pathways.