Establishment of SIAISi002-A, an induced pluripotent stem cell (iPSC) line from 39-year old healthy female donor with a family history of Alzheimer's disease (AD) and two copies of APOE4 gene

Establishment of SIAISi002-A, an induced pluripotent stem cell (iPSC) line from 39-year old healthy female donor with a family history of Alzheimer's disease (AD) and two copies of APOE4 gene

A 39-year old healthy female with a family history of Alzheimer's disease (AD) and two copies of apolipoprotein E(APOE) ε4 allele donated her Peripheral blood mononuclear cells (PBMC). The non-integrative Sendai viral vectors used to reprogram PBMCs with the human OKSM transcription factors. Th...

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Main Authors: Yi Yan, Yinghui Qiu, Aonan Zhao, Jian Zhao, Ying Wang, Yulei Deng
Format: Article
Language:English
Published: Elsevier 2020-05-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506120300957
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spelling doaj-9a0a07fc52584526bab91b6c4b6a95d02020-11-25T02:50:00ZengElsevierStem Cell Research1873-50612020-05-0145Establishment of SIAISi002-A, an induced pluripotent stem cell (iPSC) line from 39-year old healthy female donor with a family history of Alzheimer's disease (AD) and two copies of APOE4 geneYi Yan0Yinghui Qiu1Aonan Zhao2Jian Zhao3Ying Wang4Yulei Deng5Department of Neurology & Institute of Neurology, Ruijin Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Neurology & Institute of Neurology, Ruijin Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaDepartment of Neurology & Institute of Neurology, Ruijin Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, ChinaShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; School of Life Science and Technology, ShanghaiTech University, Shanghai, ChinaShanghai Institute for Advanced Immunochemical Studies, ShanghaiTech University, Shanghai, China; Corresponding authors.Department of Neurology & Institute of Neurology, Ruijin Hospital affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai, China; Corresponding authors.A 39-year old healthy female with a family history of Alzheimer's disease (AD) and two copies of apolipoprotein E(APOE) ε4 allele donated her Peripheral blood mononuclear cells (PBMC). The non-integrative Sendai viral vectors used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of iPSCs was confirmed by immunocytochemistry and ability of differentiation spontaneously into 3 germ layers. Furthermore, the iPSC line displayed a normal karyotype. The APOE gene is currently considered to be the most relevant gene for sporadic AD. Our model might offer a platform to study the pathological mechanisms and drug testing studies in AD.http://www.sciencedirect.com/science/article/pii/S1873506120300957
collection DOAJ
language English
format Article
sources DOAJ
author Yi Yan
Yinghui Qiu
Aonan Zhao
Jian Zhao
Ying Wang
Yulei Deng
spellingShingle Yi Yan
Yinghui Qiu
Aonan Zhao
Jian Zhao
Ying Wang
Yulei Deng
Establishment of SIAISi002-A, an induced pluripotent stem cell (iPSC) line from 39-year old healthy female donor with a family history of Alzheimer's disease (AD) and two copies of APOE4 gene
Stem Cell Research
author_facet Yi Yan
Yinghui Qiu
Aonan Zhao
Jian Zhao
Ying Wang
Yulei Deng
author_sort Yi Yan
title Establishment of SIAISi002-A, an induced pluripotent stem cell (iPSC) line from 39-year old healthy female donor with a family history of Alzheimer's disease (AD) and two copies of APOE4 gene
title_short Establishment of SIAISi002-A, an induced pluripotent stem cell (iPSC) line from 39-year old healthy female donor with a family history of Alzheimer's disease (AD) and two copies of APOE4 gene
title_full Establishment of SIAISi002-A, an induced pluripotent stem cell (iPSC) line from 39-year old healthy female donor with a family history of Alzheimer's disease (AD) and two copies of APOE4 gene
title_fullStr Establishment of SIAISi002-A, an induced pluripotent stem cell (iPSC) line from 39-year old healthy female donor with a family history of Alzheimer's disease (AD) and two copies of APOE4 gene
title_full_unstemmed Establishment of SIAISi002-A, an induced pluripotent stem cell (iPSC) line from 39-year old healthy female donor with a family history of Alzheimer's disease (AD) and two copies of APOE4 gene
title_sort establishment of siaisi002-a, an induced pluripotent stem cell (ipsc) line from 39-year old healthy female donor with a family history of alzheimer's disease (ad) and two copies of apoe4 gene
publisher Elsevier
series Stem Cell Research
issn 1873-5061
publishDate 2020-05-01
description A 39-year old healthy female with a family history of Alzheimer's disease (AD) and two copies of apolipoprotein E(APOE) ε4 allele donated her Peripheral blood mononuclear cells (PBMC). The non-integrative Sendai viral vectors used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of iPSCs was confirmed by immunocytochemistry and ability of differentiation spontaneously into 3 germ layers. Furthermore, the iPSC line displayed a normal karyotype. The APOE gene is currently considered to be the most relevant gene for sporadic AD. Our model might offer a platform to study the pathological mechanisms and drug testing studies in AD.
url http://www.sciencedirect.com/science/article/pii/S1873506120300957
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AT yinghuiqiu establishmentofsiaisi002aaninducedpluripotentstemcellipsclinefrom39yearoldhealthyfemaledonorwithafamilyhistoryofalzheimersdiseaseadandtwocopiesofapoe4gene
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