Establishment of SIAISi002-A, an induced pluripotent stem cell (iPSC) line from 39-year old healthy female donor with a family history of Alzheimer's disease (AD) and two copies of APOE4 gene
A 39-year old healthy female with a family history of Alzheimer's disease (AD) and two copies of apolipoprotein E(APOE) ε4 allele donated her Peripheral blood mononuclear cells (PBMC). The non-integrative Sendai viral vectors used to reprogram PBMCs with the human OKSM transcription factors. Th...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2020-05-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506120300957 |
| Summary: | A 39-year old healthy female with a family history of Alzheimer's disease (AD) and two copies of apolipoprotein E(APOE) ε4 allele donated her Peripheral blood mononuclear cells (PBMC). The non-integrative Sendai viral vectors used to reprogram PBMCs with the human OKSM transcription factors. The pluripotency of iPSCs was confirmed by immunocytochemistry and ability of differentiation spontaneously into 3 germ layers. Furthermore, the iPSC line displayed a normal karyotype. The APOE gene is currently considered to be the most relevant gene for sporadic AD. Our model might offer a platform to study the pathological mechanisms and drug testing studies in AD. |
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| ISSN: | 1873-5061 |