LPS-Inducible lncRNA TMC3-AS1 Negatively Regulates the Expression of IL-10

• Main Authors: Mengling Ye, Minghong Xie, Jie Zhu, Chen Wang, Rui Zhou, Xiaoqing Li
• Format: Article
• Language: English
• Published: Frontiers Media S.A. 2020-07-01
• Series: Frontiers in Immunology
• Subjects: NF-κB; TMC3-AS1; IL-10; innate immunity; TLR4
• Online Access: https://www.frontiersin.org/article/10.3389/fimmu.2020.01418/full

Long non-coding RNAs are essential regulators of the inflammatory response, especially for transcriptional regulation of inflammatory genes. It has been reported that the expression of transmembrane channel-like 3 (TMC3)–AS1 is increased following lipopolysaccharide stimulation. However, the potential function of TMC3-AS1 in immunity is largely unknown. Herein, we report a specific role for TMC3-AS1 in the regulation of inflammatory gene expression. TMC3-AS1 negatively regulates the expression of interleukin 10 (IL-10) in macrophage and intestinal epithelial cell lines. Mechanistically, TMC3-AS1 may interact with p65 in the nucleus, preventing p65 from binding to the κB consensus site within IL-10 promoter. These findings suggest that TMC3-AS1 may function as an important regulator in the innate immune response.