User-friendly extraction and multistage tandem mass spectrometry based analysis of lipid-linked oligosaccharides in microalgae

User-friendly extraction and multistage tandem mass spectrometry based analysis of lipid-linked oligosaccharides in microalgae

Abstract Background Protein N-glycosylation is initiated within the endoplasmic reticulum through the synthesis of a lipid-linked oligosaccharides (LLO) precursor. This precursor is then transferred en bloc on neo-synthesized proteins through the action of the oligosaccharyltransferase giving birth...

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Bibliographic Details
Main Authors: Pierre-Louis Lucas, Rodolphe Dumontier, Corinne Loutelier-Bourhis, Alain Mareck, Carlos Afonso, Patrice Lerouge, Narimane Mati-Baouche, Muriel Bardor
Format: Article
Language:English
Published: BMC 2018-12-01
Series:Plant Methods
Subjects:
Diatom
Chlamydomonas reinhardtii
Phaeodactylum tricornutum
Lipid-linked oligosaccharides
Microalgae
Multistage tandem mass spectrometry
Online Access:http://link.springer.com/article/10.1186/s13007-018-0374-8

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