Collybistin and gephyrin are novel components of the eukaryotic translation initiation factor 3 complex

• Main Authors: De Paula Vanessa J, de Alencastro Gustavo, Sertie Andrea L, Passos-Bueno Maria
• Format: Article
• Language: English
• Published: BMC 2010-09-01
• Series: BMC Research Notes
• Online Access: http://www.biomedcentral.com/1756-0500/3/242

<p>Abstract</p> <p>Background</p> <p>Collybistin (CB), a neuron-specific guanine nucleotide exchange factor, has been implicated in targeting gephyrin-GABA_Areceptors clusters to inhibitory postsynaptic sites. However, little is known about additional CB partners and functions.</p> <p>Findings</p> <p>Here, we identified the p40 subunit of the eukaryotic translation initiation factor 3 (eIF3H) as a novel binding partner of CB, documenting the interaction in yeast, non-neuronal cell lines, and the brain. In addition, we demonstrated that gephyrin also interacts with eIF3H in non-neuronal cells and forms a complex with eIF3 in the brain.</p> <p>Conclusions</p> <p>Together, our results suggest, for the first time, that CB and gephyrin associate with the translation initiation machinery, and lend further support to the previous evidence that gephyrin may act as a regulator of synaptic protein synthesis.</p>