Breakdown of lecithin on aluminum oxide columns*

A method is described for the detection of slow breakdown reactions that sometimes occur in chromatographic columns. Hydrolysis of lecithin on aluminum-oxide columns has been demonstrated to occur under conditions normally used in the chromatography of phosphatides. About 1% of lecithin is estimated...

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Main Author:	O. Renkonen
Format:	Article
Language:	English
Published:	Elsevier 1962-04-01
Series:	Journal of Lipid Research
Online Access:	http://www.sciencedirect.com/science/article/pii/S0022227520404250

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spelling	doaj-98ba382f7c4d48f6985becb11b172d092021-04-23T06:13:04ZengElsevierJournal of Lipid Research0022-22751962-04-0132181183Breakdown of lecithin on aluminum oxide columns*O. Renkonen0Department of Serology and Bacteriology, University of Helsinki, FinlandA method is described for the detection of slow breakdown reactions that sometimes occur in chromatographic columns. Hydrolysis of lecithin on aluminum-oxide columns has been demonstrated to occur under conditions normally used in the chromatography of phosphatides. About 1% of lecithin is estimated to be destroyed on alumina columns in 1 hour at 22°. A substantial part of the decomposed lecithin is found as lysolecithin. Lowering the working temperature to 2° markedly reduces the rate of the degradation of lecithin and that of the formation of lysolecithin.http://www.sciencedirect.com/science/article/pii/S0022227520404250
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	O. Renkonen
spellingShingle	O. Renkonen Breakdown of lecithin on aluminum oxide columns* Journal of Lipid Research
author_facet	O. Renkonen
author_sort	O. Renkonen
title	Breakdown of lecithin on aluminum oxide columns*
title_short	Breakdown of lecithin on aluminum oxide columns*
title_full	Breakdown of lecithin on aluminum oxide columns*
title_fullStr	Breakdown of lecithin on aluminum oxide columns*
title_full_unstemmed	Breakdown of lecithin on aluminum oxide columns*
title_sort	breakdown of lecithin on aluminum oxide columns*
publisher	Elsevier
series	Journal of Lipid Research
issn	0022-2275
publishDate	1962-04-01
description	A method is described for the detection of slow breakdown reactions that sometimes occur in chromatographic columns. Hydrolysis of lecithin on aluminum-oxide columns has been demonstrated to occur under conditions normally used in the chromatography of phosphatides. About 1% of lecithin is estimated to be destroyed on alumina columns in 1 hour at 22°. A substantial part of the decomposed lecithin is found as lysolecithin. Lowering the working temperature to 2° markedly reduces the rate of the degradation of lecithin and that of the formation of lysolecithin.
url	http://www.sciencedirect.com/science/article/pii/S0022227520404250
work_keys_str_mv	AT orenkonen breakdownoflecithinonaluminumoxidecolumns
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Breakdown of lecithin on aluminum oxide columns*

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