Breakdown of lecithin on aluminum oxide columns*
A method is described for the detection of slow breakdown reactions that sometimes occur in chromatographic columns. Hydrolysis of lecithin on aluminum-oxide columns has been demonstrated to occur under conditions normally used in the chromatography of phosphatides. About 1% of lecithin is estimated...
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| Format: | Article |
| Language: | English |
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Elsevier
1962-04-01
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| Series: | Journal of Lipid Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520404250 |
| Summary: | A method is described for the detection of slow breakdown reactions that sometimes occur in chromatographic columns. Hydrolysis of lecithin on aluminum-oxide columns has been demonstrated to occur under conditions normally used in the chromatography of phosphatides. About 1% of lecithin is estimated to be destroyed on alumina columns in 1 hour at 22°. A substantial part of the decomposed lecithin is found as lysolecithin. Lowering the working temperature to 2° markedly reduces the rate of the degradation of lecithin and that of the formation of lysolecithin. |
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| ISSN: | 0022-2275 |