Real-time quantification of protein expression at the single-cell level via dynamic protein synthesis translocation reporters

Real-time quantification of protein expression at the single-cell level via dynamic protein synthesis translocation reporters

Single cells can display large heterogeneity in gene induction. Here, Aymoz et al. present an expression reporter based on protein translocation that can accurately measure both the levels and dynamics of protein synthesis in live single cells with a temporal resolution of less than one minute.

Bibliographic Details
Main Authors: Delphine Aymoz, Victoria Wosika, Eric Durandau, Serge Pelet
Format: Article
Language:English
Published: Nature Publishing Group 2016-04-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/ncomms11304
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spelling doaj-985c22e4d7a44ff48c0c5e471df769612021-05-11T10:42:33ZengNature Publishing GroupNature Communications2041-17232016-04-017111210.1038/ncomms11304Real-time quantification of protein expression at the single-cell level via dynamic protein synthesis translocation reportersDelphine Aymoz0Victoria Wosika1Eric Durandau2Serge Pelet3Department of Fundamental Microbiology, University of LausanneDepartment of Fundamental Microbiology, University of LausanneDepartment of Fundamental Microbiology, University of LausanneDepartment of Fundamental Microbiology, University of LausanneSingle cells can display large heterogeneity in gene induction. Here, Aymoz et al. present an expression reporter based on protein translocation that can accurately measure both the levels and dynamics of protein synthesis in live single cells with a temporal resolution of less than one minute.https://doi.org/10.1038/ncomms11304
collection DOAJ
language English
format Article
sources DOAJ
author Delphine Aymoz
Victoria Wosika
Eric Durandau
Serge Pelet
spellingShingle Delphine Aymoz
Victoria Wosika
Eric Durandau
Serge Pelet
Real-time quantification of protein expression at the single-cell level via dynamic protein synthesis translocation reporters
Nature Communications
author_facet Delphine Aymoz
Victoria Wosika
Eric Durandau
Serge Pelet
author_sort Delphine Aymoz
title Real-time quantification of protein expression at the single-cell level via dynamic protein synthesis translocation reporters
title_short Real-time quantification of protein expression at the single-cell level via dynamic protein synthesis translocation reporters
title_full Real-time quantification of protein expression at the single-cell level via dynamic protein synthesis translocation reporters
title_fullStr Real-time quantification of protein expression at the single-cell level via dynamic protein synthesis translocation reporters
title_full_unstemmed Real-time quantification of protein expression at the single-cell level via dynamic protein synthesis translocation reporters
title_sort real-time quantification of protein expression at the single-cell level via dynamic protein synthesis translocation reporters
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2016-04-01
description Single cells can display large heterogeneity in gene induction. Here, Aymoz et al. present an expression reporter based on protein translocation that can accurately measure both the levels and dynamics of protein synthesis in live single cells with a temporal resolution of less than one minute.
url https://doi.org/10.1038/ncomms11304
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AT ericdurandau realtimequantificationofproteinexpressionatthesinglecelllevelviadynamicproteinsynthesistranslocationreporters
AT sergepelet realtimequantificationofproteinexpressionatthesinglecelllevelviadynamicproteinsynthesistranslocationreporters
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