Comparative evaluation of the microplate nitrate reductase assay and the rezasurin microtitre assay for the rapid detection of multidrug resistant Mycobacterium tuberculosis clinical isolates

• Main Authors: Ahmet Yilmaz Coban, Meltem Uzun, Alper Akgunes, Belma Durupinar
• Format: Article
• Language: English
• Published: Instituto Oswaldo Cruz, Ministério da Saúde 2012-08-01
• Series: Memórias do Instituto Oswaldo Cruz.
• Subjects: Mycobacterium tuberculosis; MIC determination; microplate nitrate reductase assay; rezasurin microtitre assay
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762012000500002&lng=en&tlng=en

The microplate nitrate reductase assay (MNRA) and the rezasurin microtitre assay (REMA) were used for the susceptibility testing of 73 clinical isolates and the results were compared with those that were obtained using the Bactec 460 TB and Bactec MGIT 960 systems. The REMA and the MNRA were performed in 96-well plates. For the REMA, the concentrations of isoniazid (INH) and rifampicin (RIF) ranged from 1.0-0.01 µg/mL and 2.0-0.03 µg/mL, respectively. For the MNRA, the INH concentration was between 1.0-0.03 µg/mL and the RIF concentration was between 2.0-0.06 µg/mL. For the MNRA, the sensitivity, specificity, positive predictive value, negative predictive value and INH/RIF agreement were 100/95.6, 97.6/100, 96.8/100, 100/98 and 98.6/98.6, respectively, and for the REMA, they were 100/91.3, 90.4/100, 88.5/100, 100/96.1 and 94.5/97.2, respectively. Our data suggest that these two rapid, low-cost methods may be inexpensive, alternative assays for the rapid detection of multidrug resistant tuberculosis in low-income countries.