<i>Janthinobacterium tructae</i> sp. nov., Isolated from Kidney of Rainbow Trout (<i>Oncorhynchus mykiss</i>)

• Main Authors: Won Joon Jung, Sang Wha Kim, Sib Sankar Giri, Hyoun Joong Kim, Sang Guen Kim, Jeong Woo Kang, Jun Kwon, Sung Bin Lee, Woo Taek Oh, Jin Woo Jun, Se Chang Park
• Format: Article
• Language: English
• Published: MDPI AG 2021-02-01
• Series: Pathogens
• Subjects: Jantinobacterium; Jantinobacterium tructae; rainbow trout; Oncorhynchus mykiss
• Online Access: https://www.mdpi.com/2076-0817/10/2/229

This study presents a novel <i>Janthinobacterium </i>strain, SNU WT3, isolated from the kidney of rainbow trout. A phylogenetic study using 16S rRNA sequences indicated that the strain is closely related to <i>Janthinobacterium svalbardensis</i> JA-1^T. However, biochemical analysis found differences in D-xylose adonitol, N-acetylglucosamine, arbutin, and cellobiose. As for genome-to-genome distance and average nucleotide identity values calculated between strain SNU WT3 and other related strains such as <i>J. lividum</i> EIF1, <i>J. svalbardensis</i> PAMC 27463, and <i>J. agaricidamnosum</i> BHSEK were all below the cutoff value between species. DNA-DNA hybridization between strain SNU WT3 and other close relatives indicated the results of <i>J. lividum</i> DSM 1522^T (47.11%) and <i>J. svalbardensis</i> JA-1^T (38.88%) individually. The major fatty acid compositions of strain SNU WT3 were cylco-C_17:0 (41.45%), C_16:0 (33.86%) and C_12:0 (5.87%). The major polar lipids were phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol, and diphosphatidylglycerol. The quinone system was composed mainly of ubiquinone Q-8. The genome of strain SNU WT3 consists of 6,314,370 bp with a G + C content of 62.35%. Here, we describe a novel species of the genus <i>Janthinobacterium</i>, and the name <i>Janthinobacterium tructae</i> has been proposed with SNU WT3^T (=KCTC 72518 = JCM 33613) as the type strain.