Folic Acid–Functionalized Composite Scaffolds of Gelatin and Gold Nanoparticles for Photothermal Ablation of Breast Cancer Cells

Folic Acid–Functionalized Composite Scaffolds of Gelatin and Gold Nanoparticles for Photothermal Ablation of Breast Cancer Cells

Photothermal therapy (PTT) has been developed as a useful therapeutic method for cancer treatment. Localization of PTT agents in cancer sites and targeting capacity are required to further increase therapeutic efficacy. In this study, gold nanoparticles (AuNPs) and gelatin were functionalized with f...

Full description

Bibliographic Details
Main Authors: Huajian Chen, Xiuhui Wang, Linawati Sutrisno, Tianjiao Zeng, Naoki Kawazoe, Yingnan Yang, Guoping Chen
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-11-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
photothermal therapy
folic acid
composite scaffolds
gold nanoparticles
cell targeting
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2020.589905/full
id doaj-97f719d6bb034c8b8fc6d7ef1cc0586e
record_format Article
spelling doaj-97f719d6bb034c8b8fc6d7ef1cc0586e2020-11-25T04:06:54ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-11-01810.3389/fbioe.2020.589905589905Folic Acid–Functionalized Composite Scaffolds of Gelatin and Gold Nanoparticles for Photothermal Ablation of Breast Cancer CellsHuajian Chen0Huajian Chen1Xiuhui Wang2Xiuhui Wang3Linawati Sutrisno4Linawati Sutrisno5Tianjiao Zeng6Naoki Kawazoe7Yingnan Yang8Guoping Chen9Guoping Chen10Research Center for Functional Materials, National Institute for Materials Science, Tsukuba, JapanDepartment of Materials Science and Engineering, Graduate School of Pure and Applied Sciences, University of Tsukuba, Tsukuba, JapanResearch Center for Functional Materials, National Institute for Materials Science, Tsukuba, JapanInstitute of Translational Medicine, Shanghai University, Shanghai, ChinaResearch Center for Functional Materials, National Institute for Materials Science, Tsukuba, JapanDepartment of Materials Science and Engineering, Graduate School of Pure and Applied Sciences, University of Tsukuba, Tsukuba, JapanGraduate School of Life and Environmental Science, University of Tsukuba, Tsukuba, JapanResearch Center for Functional Materials, National Institute for Materials Science, Tsukuba, JapanGraduate School of Life and Environmental Science, University of Tsukuba, Tsukuba, JapanResearch Center for Functional Materials, National Institute for Materials Science, Tsukuba, JapanDepartment of Materials Science and Engineering, Graduate School of Pure and Applied Sciences, University of Tsukuba, Tsukuba, JapanPhotothermal therapy (PTT) has been developed as a useful therapeutic method for cancer treatment. Localization of PTT agents in cancer sites and targeting capacity are required to further increase therapeutic efficacy. In this study, gold nanoparticles (AuNPs) and gelatin were functionalized with folic acid (FA) and hybridized to prepare FA-functionalized gelatin–AuNPs composite scaffolds. AuNPs with rod and star shapes of three sizes (40, 70, and 110 nm) were used for the hybridization to investigate the influence of AuNPs shape and size. The composite scaffolds showed porous structures with good interconnectivity. Modification with FA increased capture capacity of the composite scaffolds. Hybridization with AuNPs rendered the composite scaffold a good photothermal conversion property under near-infrared (NIR) laser irradiation. Temperature change during laser irradiation increased with the laser power intensity and irradiation time. The shape and size of AuNPs also affected their photothermal conversion property. The composite scaffold of gold nanorods 70 (FA-G/R70) had the highest photothermal conversion capacity. Breast cancer cells cultured in the FA-G/R70 composite scaffold were killed under NIR laser irradiation. Mouse subcutaneous implantation further demonstrated the excellent photothermal ablation capability of FA-G/R70 composite scaffold to breast cancer cells. The FA-functionalized composite scaffolds were demonstrated a high potential for local PPT of breast cancer.https://www.frontiersin.org/articles/10.3389/fbioe.2020.589905/fullphotothermal therapyfolic acidcomposite scaffoldsgold nanoparticlescell targeting
collection DOAJ
language English
format Article
sources DOAJ
author Huajian Chen
Huajian Chen
Xiuhui Wang
Xiuhui Wang
Linawati Sutrisno
Linawati Sutrisno
Tianjiao Zeng
Naoki Kawazoe
Yingnan Yang
Guoping Chen
Guoping Chen
spellingShingle Huajian Chen
Huajian Chen
Xiuhui Wang
Xiuhui Wang
Linawati Sutrisno
Linawati Sutrisno
Tianjiao Zeng
Naoki Kawazoe
Yingnan Yang
Guoping Chen
Guoping Chen
Folic Acid–Functionalized Composite Scaffolds of Gelatin and Gold Nanoparticles for Photothermal Ablation of Breast Cancer Cells
Frontiers in Bioengineering and Biotechnology
photothermal therapy
folic acid
composite scaffolds
gold nanoparticles
cell targeting
author_facet Huajian Chen
Huajian Chen
Xiuhui Wang
Xiuhui Wang
Linawati Sutrisno
Linawati Sutrisno
Tianjiao Zeng
Naoki Kawazoe
Yingnan Yang
Guoping Chen
Guoping Chen
author_sort Huajian Chen
title Folic Acid–Functionalized Composite Scaffolds of Gelatin and Gold Nanoparticles for Photothermal Ablation of Breast Cancer Cells
title_short Folic Acid–Functionalized Composite Scaffolds of Gelatin and Gold Nanoparticles for Photothermal Ablation of Breast Cancer Cells
title_full Folic Acid–Functionalized Composite Scaffolds of Gelatin and Gold Nanoparticles for Photothermal Ablation of Breast Cancer Cells
title_fullStr Folic Acid–Functionalized Composite Scaffolds of Gelatin and Gold Nanoparticles for Photothermal Ablation of Breast Cancer Cells
title_full_unstemmed Folic Acid–Functionalized Composite Scaffolds of Gelatin and Gold Nanoparticles for Photothermal Ablation of Breast Cancer Cells
title_sort folic acid–functionalized composite scaffolds of gelatin and gold nanoparticles for photothermal ablation of breast cancer cells
publisher Frontiers Media S.A.
series Frontiers in Bioengineering and Biotechnology
issn 2296-4185
publishDate 2020-11-01
description Photothermal therapy (PTT) has been developed as a useful therapeutic method for cancer treatment. Localization of PTT agents in cancer sites and targeting capacity are required to further increase therapeutic efficacy. In this study, gold nanoparticles (AuNPs) and gelatin were functionalized with folic acid (FA) and hybridized to prepare FA-functionalized gelatin–AuNPs composite scaffolds. AuNPs with rod and star shapes of three sizes (40, 70, and 110 nm) were used for the hybridization to investigate the influence of AuNPs shape and size. The composite scaffolds showed porous structures with good interconnectivity. Modification with FA increased capture capacity of the composite scaffolds. Hybridization with AuNPs rendered the composite scaffold a good photothermal conversion property under near-infrared (NIR) laser irradiation. Temperature change during laser irradiation increased with the laser power intensity and irradiation time. The shape and size of AuNPs also affected their photothermal conversion property. The composite scaffold of gold nanorods 70 (FA-G/R70) had the highest photothermal conversion capacity. Breast cancer cells cultured in the FA-G/R70 composite scaffold were killed under NIR laser irradiation. Mouse subcutaneous implantation further demonstrated the excellent photothermal ablation capability of FA-G/R70 composite scaffold to breast cancer cells. The FA-functionalized composite scaffolds were demonstrated a high potential for local PPT of breast cancer.
topic photothermal therapy
folic acid
composite scaffolds
gold nanoparticles
cell targeting
url https://www.frontiersin.org/articles/10.3389/fbioe.2020.589905/full
work_keys_str_mv AT huajianchen folicacidfunctionalizedcompositescaffoldsofgelatinandgoldnanoparticlesforphotothermalablationofbreastcancercells
AT huajianchen folicacidfunctionalizedcompositescaffoldsofgelatinandgoldnanoparticlesforphotothermalablationofbreastcancercells
AT xiuhuiwang folicacidfunctionalizedcompositescaffoldsofgelatinandgoldnanoparticlesforphotothermalablationofbreastcancercells
AT xiuhuiwang folicacidfunctionalizedcompositescaffoldsofgelatinandgoldnanoparticlesforphotothermalablationofbreastcancercells
AT linawatisutrisno folicacidfunctionalizedcompositescaffoldsofgelatinandgoldnanoparticlesforphotothermalablationofbreastcancercells
AT linawatisutrisno folicacidfunctionalizedcompositescaffoldsofgelatinandgoldnanoparticlesforphotothermalablationofbreastcancercells
AT tianjiaozeng folicacidfunctionalizedcompositescaffoldsofgelatinandgoldnanoparticlesforphotothermalablationofbreastcancercells
AT naokikawazoe folicacidfunctionalizedcompositescaffoldsofgelatinandgoldnanoparticlesforphotothermalablationofbreastcancercells
AT yingnanyang folicacidfunctionalizedcompositescaffoldsofgelatinandgoldnanoparticlesforphotothermalablationofbreastcancercells
AT guopingchen folicacidfunctionalizedcompositescaffoldsofgelatinandgoldnanoparticlesforphotothermalablationofbreastcancercells
AT guopingchen folicacidfunctionalizedcompositescaffoldsofgelatinandgoldnanoparticlesforphotothermalablationofbreastcancercells
_version_ 1724430299509555200

Similar Items