Human endometrial cell coculture reduces the endocrine disruptor toxicity on mouse embryo development

• Main Authors: Lee Myeong-Seop, Lee Young-Sang, Lee Hae-Hyeog, Song Ho-Yeon
• Format: Article
• Language: English
• Published: BMC 2012-04-01
• Series: Journal of Occupational Medicine and Toxicology
• Subjects: Bisphenol A; Aroclor 1254; Mouse embryo; Human endometrial cells; Endocrine disruptors
• Online Access: http://www.occup-med.com/content/7/1/7

<p>Abstract</p> <p>Backgrounds</p> <p>Previous studies suggested that endocrine disruptors (ED) are toxic on preimplantation embryos and inhibit development of embryos <it>in vitro</it> culture. However, information about the toxicity of endocrine disruptors on preimplantation development of embryo in human reproductive environment is lacking.</p> <p>Methods</p> <p>Bisphenol A (BPA) and Aroclor 1254 (polychlorinated biphenyls) were used as endocrine disruptors in this study. Mouse 2-cell embryos were cultured in medium alone or vehicle or co-cultured with human endometrial epithelial layers in increasing ED concentrations.</p> <p>Results</p> <p>At 72 hours the percentage of normal blastocyst were decreased by ED in a dose-dependent manner while the co-culture system significantly enhanced the rate and reduced the toxicity of endocrine disruptors on the embryonic development <it>in vitro</it>.</p> <p>Conclusions</p> <p>In conclusion, although EDs have the toxic effect on embryo development, the co-culture with human endometrial cell reduced the preimplantation embryo from it thereby making human reproductive environment protective to preimplantation embryo from the toxicity of endocrine disruptors.</p>