Bridging the gap between reference and real transcriptomes

Bridging the gap between reference and real transcriptomes

Abstract Genetic, transcriptional, and post-transcriptional variations shape the transcriptome of individual cells, rendering establishing an exhaustive set of reference RNAs a complicated matter. Current reference transcriptomes, which are based on carefully curated transcripts, are lagging behind...

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Main Authors: Antonin Morillon, Daniel Gautheret
Format: Article
Language:English
Published: BMC 2019-06-01
Series:Genome Biology
Online Access:http://link.springer.com/article/10.1186/s13059-019-1710-7
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spelling doaj-97dcd0292ff84de1b7d21965532da6ba2020-11-25T03:29:46ZengBMCGenome Biology1474-760X2019-06-012011710.1186/s13059-019-1710-7Bridging the gap between reference and real transcriptomesAntonin Morillon0Daniel Gautheret1ncRNA, Epigenetic and Genome Fluidity, CNRS UMR 3244, Sorbonne Université, PSL University, Institut Curie, Centre de RechercheInstitute for Integrative Biology of the Cell, CEA, CNRS, Université Paris-Sud, Université Paris SaclayAbstract Genetic, transcriptional, and post-transcriptional variations shape the transcriptome of individual cells, rendering establishing an exhaustive set of reference RNAs a complicated matter. Current reference transcriptomes, which are based on carefully curated transcripts, are lagging behind the extensive RNA variation revealed by massively parallel sequencing. Much may be missed by ignoring this unreferenced RNA diversity. There is plentiful evidence for non-reference transcripts with important phenotypic effects. Although reference transcriptomes are inestimable for gene expression analysis, they may turn limiting in important medical applications. We discuss computational strategies for retrieving hidden transcript diversity.http://link.springer.com/article/10.1186/s13059-019-1710-7
collection DOAJ
language English
format Article
sources DOAJ
author Antonin Morillon
Daniel Gautheret
spellingShingle Antonin Morillon
Daniel Gautheret
Bridging the gap between reference and real transcriptomes
Genome Biology
author_facet Antonin Morillon
Daniel Gautheret
author_sort Antonin Morillon
title Bridging the gap between reference and real transcriptomes
title_short Bridging the gap between reference and real transcriptomes
title_full Bridging the gap between reference and real transcriptomes
title_fullStr Bridging the gap between reference and real transcriptomes
title_full_unstemmed Bridging the gap between reference and real transcriptomes
title_sort bridging the gap between reference and real transcriptomes
publisher BMC
series Genome Biology
issn 1474-760X
publishDate 2019-06-01
description Abstract Genetic, transcriptional, and post-transcriptional variations shape the transcriptome of individual cells, rendering establishing an exhaustive set of reference RNAs a complicated matter. Current reference transcriptomes, which are based on carefully curated transcripts, are lagging behind the extensive RNA variation revealed by massively parallel sequencing. Much may be missed by ignoring this unreferenced RNA diversity. There is plentiful evidence for non-reference transcripts with important phenotypic effects. Although reference transcriptomes are inestimable for gene expression analysis, they may turn limiting in important medical applications. We discuss computational strategies for retrieving hidden transcript diversity.
url http://link.springer.com/article/10.1186/s13059-019-1710-7
work_keys_str_mv AT antoninmorillon bridgingthegapbetweenreferenceandrealtranscriptomes
AT danielgautheret bridgingthegapbetweenreferenceandrealtranscriptomes
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