The effect of Spirulina algae on the immune response of SPF chickens to commercial inactivated Newcastle vaccine in poultry

• Main Authors: Mahmoud M. Abotaleb, Mohamed S. Abousenna, Amr M. Helal, Samir A. Nassif, Mounir M. Elsafty
• Format: Article
• Language: English
• Published: Finlay Ediciones 2020-06-01
• Series: VacciMonitor
• Subjects: spirulina; newcastle disease virus; humoral immune response; real-time pcr; vaccination; serological tests
• Online Access: http://scielo.sld.cu/scielo.php?script=sci_arttext&pid=S1025-028X2020000200051&lng=es&nrm=iso&tlng=en
• ISSN: 1025-028X; 1025-0298

The objective of this study was to investigate the effects of Spirulina platensis (SP) powder supplementation on immune response in SPF chickens. For this purpose, 120 SPF chicks were randomly clustered into six groups consisting of 20 birds each which assigned to five groups vaccinated by commercial inactivated Newcastle disease (ND) vaccine at 21 days of age. The four groups were supplemented with 0.5, 1, 1.5 and 2 g of SP per kg of ration at 7 day of age and other group as control treatment group. Control unvaccinated group still without any treatment. Individual blood samples were collected weekly from all groups, and NDV-HI antibodies were measured using Hemagglutination inhibition (HI) test. After 28 days post-vaccination, ten birds from all groups were challenged intramuscularly at a dose 0.5 mL/bird containing 106 EID50 of local NDV genotype VII. Challenge virus shedding was detected using real time qrt-PCR of oropharyngeal swabs that were collected from all challenged chicken groups of at 3, 5, 7 and 10 days post challenge. Obtained results showed that vaccinated groups of SPF-chickens either supplied with Spirulina or control treatment group induced positive serological response as NDV-HI antibody were measured in sera of immunized chicks (7.6, 8, 8.3, 8.9 and 7.4 log2, respectively) at 4 weeks post vaccination (WPV). Significant differences were observed at 2 WPV in the vaccinated SPF chickens consumed 1, 1.5 and 2 g of SP/kg of ration, compared to untreated vaccinated group (p<0.05). Immunized SPF chickens supplied with different SP concentration confer satisfactory protection against heterologous challenge virus (90%, 100%, 100% and 100% respectively), in contrast to untreated vaccinated chickens. Different percentages of reduction of viral shedding (55%, 65%, 76% and 87%) of treated vaccinated chickens with different concentration of SP were detected, despite untreated group were reduced 46% from total viral shedding. These findings suggest that dietary Spirulina has immune-stimulatory effects on the immune system of SPF chickens. One gram from SP per kg of ration was minimum recommended concentration that able to exhibit optimum immune response, increase protection against heterologous strains and able to reduce viral shedding.