Vitrification for cryopreservation of 2D and 3D stem cells culture using high concentration of cryoprotective agents

Vitrification for cryopreservation of 2D and 3D stem cells culture using high concentration of cryoprotective agents

Abstract Background Vitrification is the most promising technology for successful cryopreservation of living organisms without ice crystal formation. However, high concentrations (up to ~ 6–8 M) of cryoprotective agents (CPAs) used in stem cell induce osmotic and metabolic injuries. Moreover, the ap...

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Bibliographic Details
Main Authors: Young-Hoon Jeong, Ukjin Kim, Seul-Gi Lee, Bokyeong Ryu, Jin Kim, Artyuhov Igor, Jong Soo Kim, Cho-Rok Jung, Jae-Hak Park, C-Yoon Kim
Format: Article
Language:English
Published: BMC 2020-08-01
Series:BMC Biotechnology
Subjects:
Vitrification
Cryoprotective agent
In vitro
MSC
Spheroid
Online Access:http://link.springer.com/article/10.1186/s12896-020-00636-9

Internet

http://link.springer.com/article/10.1186/s12896-020-00636-9

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