Post-weaning Exposure to High-Fat Diet Induces Kidney Lipid Accumulation and Function Impairment in Adult Rats
Aim: We investigated the kidney morphofunctional consequences of high-fat diet intake since post-weaning in adult rats.Main Methods: Male Wistar rats were divided into two groups: ND (normal diet; n = 10) and HD (high-fat diet; n = 10). The high-fat diet was introduced post-weaned and animals were f...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-05-01
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| Series: | Frontiers in Nutrition |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fnut.2019.00060/full |
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Article |
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DOAJ |
| language |
English |
| format |
Article |
| sources |
DOAJ |
| author |
Cynthia R. Muller Cynthia R. Muller Ana Paula O. Leite Ana Paula O. Leite Rodrigo Yokota Renata O. Pereira Renata O. Pereira Anna Laura V. Americo Anna Laura V. Americo Nilberto R. F. Nascimento Fabiana S. Evangelista Vera Farah Vera Farah Manasses C. Fonteles Manasses C. Fonteles Patricia Fiorino |
| spellingShingle |
Cynthia R. Muller Cynthia R. Muller Ana Paula O. Leite Ana Paula O. Leite Rodrigo Yokota Renata O. Pereira Renata O. Pereira Anna Laura V. Americo Anna Laura V. Americo Nilberto R. F. Nascimento Fabiana S. Evangelista Vera Farah Vera Farah Manasses C. Fonteles Manasses C. Fonteles Patricia Fiorino Post-weaning Exposure to High-Fat Diet Induces Kidney Lipid Accumulation and Function Impairment in Adult Rats Frontiers in Nutrition post-weaning high-fat diet kidney lipid deposition lipotoxicity inflammation |
| author_facet |
Cynthia R. Muller Cynthia R. Muller Ana Paula O. Leite Ana Paula O. Leite Rodrigo Yokota Renata O. Pereira Renata O. Pereira Anna Laura V. Americo Anna Laura V. Americo Nilberto R. F. Nascimento Fabiana S. Evangelista Vera Farah Vera Farah Manasses C. Fonteles Manasses C. Fonteles Patricia Fiorino |
| author_sort |
Cynthia R. Muller |
| title |
Post-weaning Exposure to High-Fat Diet Induces Kidney Lipid Accumulation and Function Impairment in Adult Rats |
| title_short |
Post-weaning Exposure to High-Fat Diet Induces Kidney Lipid Accumulation and Function Impairment in Adult Rats |
| title_full |
Post-weaning Exposure to High-Fat Diet Induces Kidney Lipid Accumulation and Function Impairment in Adult Rats |
| title_fullStr |
Post-weaning Exposure to High-Fat Diet Induces Kidney Lipid Accumulation and Function Impairment in Adult Rats |
| title_full_unstemmed |
Post-weaning Exposure to High-Fat Diet Induces Kidney Lipid Accumulation and Function Impairment in Adult Rats |
| title_sort |
post-weaning exposure to high-fat diet induces kidney lipid accumulation and function impairment in adult rats |
| publisher |
Frontiers Media S.A. |
| series |
Frontiers in Nutrition |
| issn |
2296-861X |
| publishDate |
2019-05-01 |
| description |
Aim: We investigated the kidney morphofunctional consequences of high-fat diet intake since post-weaning in adult rats.Main Methods: Male Wistar rats were divided into two groups: ND (normal diet; n = 10) and HD (high-fat diet; n = 10). The high-fat diet was introduced post-weaned and animals were followed for 8 weeks.Key Findings: HD group did not change body weight gain even though food consumption has decreased with no changes in caloric consumption. The HD group showed glucose intolerance and insulin resistance. The glomerular filtration rate (GFR) was decreased in vivo (ND: 2.8 ± 1.01; HD: 1.1 ± 0.14 ml/min) and in the isolated perfusion method (34% of decrease). Renal histological analysis showed a retraction in glomeruli and an increase in kidney lipid deposition (ND: 1.5 ± 0.17 HD: 5.9 ± 0.06%). Furthermore, the high-fat diet consumption increased the pro-inflammatory cytokines IL-6 (ND: 1,276 ± 203; HD: 1,982 ± 47 pg/mL/mg) and IL-1b (ND: 97 ± 12 HD: 133 ± 5 pg/mL/mg) without changing anti-inflammatory cytokine IL-10.Significance: Our study provides evidence that high-fat diet consumption leads to renal lipid accumulation, increases inflammatory cytokines, induces glomeruli retraction, and renal dysfunction. These damages observed in the kidney could be associated with an increased risk to advanced CKD in adulthood suggesting that reduction of high-fat ingestion during an early period of life can prevent metabolic disturbances and renal lipotoxicity. |
| topic |
post-weaning high-fat diet kidney lipid deposition lipotoxicity inflammation |
| url |
https://www.frontiersin.org/article/10.3389/fnut.2019.00060/full |
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doaj-97a0c560876448458c4f9ac2d1419af02020-11-25T01:19:21ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2019-05-01610.3389/fnut.2019.00060444921Post-weaning Exposure to High-Fat Diet Induces Kidney Lipid Accumulation and Function Impairment in Adult RatsCynthia R. Muller0Cynthia R. Muller1Ana Paula O. Leite2Ana Paula O. Leite3Rodrigo Yokota4Renata O. Pereira5Renata O. Pereira6Anna Laura V. Americo7Anna Laura V. Americo8Nilberto R. F. Nascimento9Fabiana S. Evangelista10Vera Farah11Vera Farah12Manasses C. Fonteles13Manasses C. Fonteles14Patricia Fiorino15Renal, Cardiovascular and Metabolic Physiopharmacology Laboratory, Health and Biological Science Center, Mackenzie Presbyterian University, São Paulo, BrazilExperimental Pathophysiology Department, Faculty of Medicine, University of São Paulo, São Paulo, BrazilRenal, Cardiovascular and Metabolic Physiopharmacology Laboratory, Health and Biological Science Center, Mackenzie Presbyterian University, São Paulo, BrazilDepartment of Medicine, Renal Division, Federal University of São Paulo, São Paulo, BrazilDepartment of Medicine, Renal Division, Federal University of São Paulo, São Paulo, BrazilRenal, Cardiovascular and Metabolic Physiopharmacology Laboratory, Health and Biological Science Center, Mackenzie Presbyterian University, São Paulo, BrazilDepartment of Medicine, Translational Medicine Division, Federal University of São Paulo, São Paulo, BrazilRenal, Cardiovascular and Metabolic Physiopharmacology Laboratory, Health and Biological Science Center, Mackenzie Presbyterian University, São Paulo, BrazilExperimental Pathophysiology Department, Faculty of Medicine, University of São Paulo, São Paulo, BrazilSuperior Institute of Biomedical Sciences, Ceara State University, Fortaleza, BrazilSchool of Arts, Science and Humanities, University of Sao Paulo, São Paulo, BrazilRenal, Cardiovascular and Metabolic Physiopharmacology Laboratory, Health and Biological Science Center, Mackenzie Presbyterian University, São Paulo, BrazilDepartment of Medicine, Translational Medicine Division, Federal University of São Paulo, São Paulo, BrazilRenal, Cardiovascular and Metabolic Physiopharmacology Laboratory, Health and Biological Science Center, Mackenzie Presbyterian University, São Paulo, BrazilSuperior Institute of Biomedical Sciences, Ceara State University, Fortaleza, BrazilRenal, Cardiovascular and Metabolic Physiopharmacology Laboratory, Health and Biological Science Center, Mackenzie Presbyterian University, São Paulo, BrazilAim: We investigated the kidney morphofunctional consequences of high-fat diet intake since post-weaning in adult rats.Main Methods: Male Wistar rats were divided into two groups: ND (normal diet; n = 10) and HD (high-fat diet; n = 10). The high-fat diet was introduced post-weaned and animals were followed for 8 weeks.Key Findings: HD group did not change body weight gain even though food consumption has decreased with no changes in caloric consumption. The HD group showed glucose intolerance and insulin resistance. The glomerular filtration rate (GFR) was decreased in vivo (ND: 2.8 ± 1.01; HD: 1.1 ± 0.14 ml/min) and in the isolated perfusion method (34% of decrease). Renal histological analysis showed a retraction in glomeruli and an increase in kidney lipid deposition (ND: 1.5 ± 0.17 HD: 5.9 ± 0.06%). Furthermore, the high-fat diet consumption increased the pro-inflammatory cytokines IL-6 (ND: 1,276 ± 203; HD: 1,982 ± 47 pg/mL/mg) and IL-1b (ND: 97 ± 12 HD: 133 ± 5 pg/mL/mg) without changing anti-inflammatory cytokine IL-10.Significance: Our study provides evidence that high-fat diet consumption leads to renal lipid accumulation, increases inflammatory cytokines, induces glomeruli retraction, and renal dysfunction. These damages observed in the kidney could be associated with an increased risk to advanced CKD in adulthood suggesting that reduction of high-fat ingestion during an early period of life can prevent metabolic disturbances and renal lipotoxicity.https://www.frontiersin.org/article/10.3389/fnut.2019.00060/fullpost-weaninghigh-fat dietkidney lipid depositionlipotoxicityinflammation |