Transcriptome analysis of differentially expressed genes involved in selenium accumulation in tea plant (Camellia sinensis).

Tea plant (Camellia sinensis) has strong enrichment ability for selenium (Se). Selenite is the main form of Se absorbed and utilized by tea plant. However, the mechanism of selenite absorption and accumulation in tea plant is still unknown. In this study, RNA sequencing (RNA-seq) was used to perform...

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Main Authors: Dan Cao, Yanli Liu, Linlong Ma, Xiaofang Jin, Guiyi Guo, Rongrong Tan, Zheng Liu, Lin Zheng, Fei Ye, Wei Liu
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5983420?pdf=render
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spelling doaj-979be6dffca34add880cd734913095be2020-11-25T00:57:39ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-01136e019750610.1371/journal.pone.0197506Transcriptome analysis of differentially expressed genes involved in selenium accumulation in tea plant (Camellia sinensis).Dan CaoYanli LiuLinlong MaXiaofang JinGuiyi GuoRongrong TanZheng LiuLin ZhengFei YeWei LiuTea plant (Camellia sinensis) has strong enrichment ability for selenium (Se). Selenite is the main form of Se absorbed and utilized by tea plant. However, the mechanism of selenite absorption and accumulation in tea plant is still unknown. In this study, RNA sequencing (RNA-seq) was used to perform transcriptomic analysis on the molecular mechanism of selenite absorption and accumulation in tea plant. 397.98 million high-quality reads were obtained and assembled into 168,212 unigenes, 89,605 of which were extensively annotated. There were 60,582 and 1,362 differentially expressed genes (DEGs) in roots and leaves, respectively. RNA-seq results were further validated by quantitative RT-PCR. Based on GO terms, the unigenes were mainly involved in cell, binding and metabolic process. KEGG pathway enrichment analysis showed that predominant pathways included ribosome and protein processing in endoplasmic reticulum. Further analysis revealed that sulfur metabolism, glutathione metabolism, selenocompound metabolism and plant hormone signal transduction responded to selenite in tea plant. Additionally, a large number of genes of higher expressions associated with phosphate transporters, sulfur assimilation, antioxidant enzymes, antioxidant substances and responses to ethylene and jasmonic acid were identified. Stress-related plant hormones might play a signaling role in promoting sulfate/selenite uptake and assimilation in tea plant. Moreover, some other Se accumulation mechanisms of tea plant were found. Our study provides a possibility for controlling Se accumulation in tea plant through bio-technologies and will be helpful for breeding new tea cultivars.http://europepmc.org/articles/PMC5983420?pdf=render
collection DOAJ
language English
format Article
sources DOAJ
author Dan Cao
Yanli Liu
Linlong Ma
Xiaofang Jin
Guiyi Guo
Rongrong Tan
Zheng Liu
Lin Zheng
Fei Ye
Wei Liu
spellingShingle Dan Cao
Yanli Liu
Linlong Ma
Xiaofang Jin
Guiyi Guo
Rongrong Tan
Zheng Liu
Lin Zheng
Fei Ye
Wei Liu
Transcriptome analysis of differentially expressed genes involved in selenium accumulation in tea plant (Camellia sinensis).
PLoS ONE
author_facet Dan Cao
Yanli Liu
Linlong Ma
Xiaofang Jin
Guiyi Guo
Rongrong Tan
Zheng Liu
Lin Zheng
Fei Ye
Wei Liu
author_sort Dan Cao
title Transcriptome analysis of differentially expressed genes involved in selenium accumulation in tea plant (Camellia sinensis).
title_short Transcriptome analysis of differentially expressed genes involved in selenium accumulation in tea plant (Camellia sinensis).
title_full Transcriptome analysis of differentially expressed genes involved in selenium accumulation in tea plant (Camellia sinensis).
title_fullStr Transcriptome analysis of differentially expressed genes involved in selenium accumulation in tea plant (Camellia sinensis).
title_full_unstemmed Transcriptome analysis of differentially expressed genes involved in selenium accumulation in tea plant (Camellia sinensis).
title_sort transcriptome analysis of differentially expressed genes involved in selenium accumulation in tea plant (camellia sinensis).
publisher Public Library of Science (PLoS)
series PLoS ONE
issn 1932-6203
publishDate 2018-01-01
description Tea plant (Camellia sinensis) has strong enrichment ability for selenium (Se). Selenite is the main form of Se absorbed and utilized by tea plant. However, the mechanism of selenite absorption and accumulation in tea plant is still unknown. In this study, RNA sequencing (RNA-seq) was used to perform transcriptomic analysis on the molecular mechanism of selenite absorption and accumulation in tea plant. 397.98 million high-quality reads were obtained and assembled into 168,212 unigenes, 89,605 of which were extensively annotated. There were 60,582 and 1,362 differentially expressed genes (DEGs) in roots and leaves, respectively. RNA-seq results were further validated by quantitative RT-PCR. Based on GO terms, the unigenes were mainly involved in cell, binding and metabolic process. KEGG pathway enrichment analysis showed that predominant pathways included ribosome and protein processing in endoplasmic reticulum. Further analysis revealed that sulfur metabolism, glutathione metabolism, selenocompound metabolism and plant hormone signal transduction responded to selenite in tea plant. Additionally, a large number of genes of higher expressions associated with phosphate transporters, sulfur assimilation, antioxidant enzymes, antioxidant substances and responses to ethylene and jasmonic acid were identified. Stress-related plant hormones might play a signaling role in promoting sulfate/selenite uptake and assimilation in tea plant. Moreover, some other Se accumulation mechanisms of tea plant were found. Our study provides a possibility for controlling Se accumulation in tea plant through bio-technologies and will be helpful for breeding new tea cultivars.
url http://europepmc.org/articles/PMC5983420?pdf=render
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