Bacterial expression of larval peritrophins of Chryosomya bezziana

• Main Authors: Tony Voucoloco, Sri Muharsini, Florentina Supriyanti, Gene Wijffels
• Format: Article
• Language: English
• Published: Pusat Penelitian dan Pengembangan Peternakan 2000-10-01
• Series: Jurnal Ilmu Ternak dan Veteriner
• Subjects: Chrysomya; peritrophic membrane; peritrophin; recombinant protein; hexaHis tag; GST
• Online Access: http://medpub.litbang.pertanian.go.id/index.php/jitv/article/view/196/196
• ISSN: 0853-7380; 2252-696X

Three candidate antigens, Chrysomya bezziana peritrophin-48, Chrysomya bezziana peritrophin-15 and Chrysomya bezziana peritrophin-42, were prepared for recombinant protein production in Escherichia coli using a variety of expression vectors. Cb peritrophin-48 was expressed as a recombinant protein possessing a carboxy-terminal hexaHis tag. Cb peritrophin-15 was expressed as both a glutathione S-transferase fusion protein and as an amino-terminal hexaHis tagged protein. The glutathione Stransferase Cb peritrophin-15 construct produced a heterogeneous group of fusion proteins. Cb peritrophin-42 was also expressed as an amino-terminal hexaHis tagged protein. The two putative domains of Cb peritrophin-42 were also separately expressed, again with amino-terminal hexaHis tags. Cultures of the hexaHis constructs Cb peritrophin-48, -15 and –42 were demonstrated to be useful for the production and purification of these protein antigens and were scaled-up for vaccine trials and protein characterization studies.