High throughput pMHC-I tetramer library production using chaperone-mediated peptide exchange

Peptide-MHC (pMHC) tetramers are important tools for probing T cell repertoire and adaptive immune responses. Here the authors use a molecular chaperone, TAPBPR, to develop a high-throughput, multiplexible platform for pMHC tetramer generation to facilitate simultaneous assessments of T cell reperto...

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Main Authors:	Sarah A. Overall, Jugmohit S. Toor, Stephanie Hao, Mark Yarmarkovich, Sara M. O’Rourke, Giora I. Morozov, Son Nguyen, Alberto Sada Japp, Nicolas Gonzalez, Danai Moschidi, Michael R. Betts, John M. Maris, Peter Smibert, Nikolaos G. Sgourakis
Format:	Article
Language:	English
Published:	Nature Publishing Group 2020-04-01
Series:	Nature Communications
Online Access:	https://doi.org/10.1038/s41467-020-15710-1

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spelling	doaj-975b9e150895476f87a40ab18d6ce5732021-05-11T09:17:23ZengNature Publishing GroupNature Communications2041-17232020-04-0111111310.1038/s41467-020-15710-1High throughput pMHC-I tetramer library production using chaperone-mediated peptide exchangeSarah A. Overall0Jugmohit S. Toor1Stephanie Hao2Mark Yarmarkovich3Sara M. O’Rourke4Giora I. Morozov5Son Nguyen6Alberto Sada Japp7Nicolas Gonzalez8Danai Moschidi9Michael R. Betts10John M. Maris11Peter Smibert12Nikolaos G. Sgourakis13Department of Chemistry and Biochemistry, University of California Santa CruzDepartment of Chemistry and Biochemistry, University of California Santa CruzTechnology Innovation Lab, New York Genome CenterDivision of Oncology, Center for Childhood Cancer Research, Children’s Hospital of Philadelphia and Department of Pediatrics, Perelman School of Medicine, University of PennsylvaniaDepartment of Chemistry and Biochemistry, University of California Santa CruzDepartment of Chemistry and Biochemistry, University of California Santa CruzDepartment of Microbiology, Perelman School of Medicine, University of PennsylvaniaDepartment of Microbiology, Perelman School of Medicine, University of PennsylvaniaDepartment of Chemistry and Biochemistry, University of California Santa CruzDepartment of Chemistry and Biochemistry, University of California Santa CruzDepartment of Microbiology, Perelman School of Medicine, University of PennsylvaniaDivision of Oncology, Center for Childhood Cancer Research, Children’s Hospital of Philadelphia and Department of Pediatrics, Perelman School of Medicine, University of PennsylvaniaTechnology Innovation Lab, New York Genome CenterDepartment of Chemistry and Biochemistry, University of California Santa CruzPeptide-MHC (pMHC) tetramers are important tools for probing T cell repertoire and adaptive immune responses. Here the authors use a molecular chaperone, TAPBPR, to develop a high-throughput, multiplexible platform for pMHC tetramer generation to facilitate simultaneous assessments of T cell repertoire/antigen specificity and transcriptome.https://doi.org/10.1038/s41467-020-15710-1
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Sarah A. Overall Jugmohit S. Toor Stephanie Hao Mark Yarmarkovich Sara M. O’Rourke Giora I. Morozov Son Nguyen Alberto Sada Japp Nicolas Gonzalez Danai Moschidi Michael R. Betts John M. Maris Peter Smibert Nikolaos G. Sgourakis
spellingShingle	Sarah A. Overall Jugmohit S. Toor Stephanie Hao Mark Yarmarkovich Sara M. O’Rourke Giora I. Morozov Son Nguyen Alberto Sada Japp Nicolas Gonzalez Danai Moschidi Michael R. Betts John M. Maris Peter Smibert Nikolaos G. Sgourakis High throughput pMHC-I tetramer library production using chaperone-mediated peptide exchange Nature Communications
author_facet	Sarah A. Overall Jugmohit S. Toor Stephanie Hao Mark Yarmarkovich Sara M. O’Rourke Giora I. Morozov Son Nguyen Alberto Sada Japp Nicolas Gonzalez Danai Moschidi Michael R. Betts John M. Maris Peter Smibert Nikolaos G. Sgourakis
author_sort	Sarah A. Overall
title	High throughput pMHC-I tetramer library production using chaperone-mediated peptide exchange
title_short	High throughput pMHC-I tetramer library production using chaperone-mediated peptide exchange
title_full	High throughput pMHC-I tetramer library production using chaperone-mediated peptide exchange
title_fullStr	High throughput pMHC-I tetramer library production using chaperone-mediated peptide exchange
title_full_unstemmed	High throughput pMHC-I tetramer library production using chaperone-mediated peptide exchange
title_sort	high throughput pmhc-i tetramer library production using chaperone-mediated peptide exchange
publisher	Nature Publishing Group
series	Nature Communications
issn	2041-1723
publishDate	2020-04-01
description	Peptide-MHC (pMHC) tetramers are important tools for probing T cell repertoire and adaptive immune responses. Here the authors use a molecular chaperone, TAPBPR, to develop a high-throughput, multiplexible platform for pMHC tetramer generation to facilitate simultaneous assessments of T cell repertoire/antigen specificity and transcriptome.
url	https://doi.org/10.1038/s41467-020-15710-1
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High throughput pMHC-I tetramer library production using chaperone-mediated peptide exchange

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