Association of interleukin-6 and its -174G/C promoter polymorphism with clinical and laboratory characteristics of non hepatitis C virus rheumatoid arthritis patients
Background: Interleukin-6 is a cytokine protein, which causes inflammation, maintains immune homeostasis and shows a role in rheumatoid arthritis pathogenesis. IL-6-174G/C promoter polymorphism may have a role in susceptibility to RA. Aim of the work: To evaluate the clinical significance of serum l...
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doaj-9758295955b14c8d9679fdc5b383cd4a2020-11-25T02:12:50ZengSpringerOpenEgyptian Journal of Medical Human Genetics1110-86302018-07-01193235240Association of interleukin-6 and its -174G/C promoter polymorphism with clinical and laboratory characteristics of non hepatitis C virus rheumatoid arthritis patientsReham Mohamed Raafat Hamed0Soliman Aref Mohamed1Reham Ali Dwedar2Yasmine Samy Elkholy3Fatema Talaat Elgengehy4Corresponding author at: 6 Gamal Abdl Nasser st from el Kawther st from Faisl st GIZA (8th floor), Egypt.; Faculty of Medicine, Cairo University, EgyptFaculty of Medicine, Cairo University, EgyptFaculty of Medicine, Cairo University, EgyptFaculty of Medicine, Cairo University, EgyptFaculty of Medicine, Cairo University, EgyptBackground: Interleukin-6 is a cytokine protein, which causes inflammation, maintains immune homeostasis and shows a role in rheumatoid arthritis pathogenesis. IL-6-174G/C promoter polymorphism may have a role in susceptibility to RA. Aim of the work: To evaluate the clinical significance of serum levels of IL-6 and its -174G/C promoter polymorphism in RA patients in comparison with the controls. Patients and methods: This study enrolled 25 non hepatitis C virus RA patients versus 25 age and gender matched controls. Demographic, clinical and laboratory data were prospectively evaluated. Serum IL-6 level and promoter (-174G/C) genotype were determined. Results: Serum IL-6 levels was significantly higher in RA patients compared to control subjects (p = .001), especially those with CC promoter polymorphism. There was a significant correlation between IL and 6 level and duration of morning stiffness, disease activity, hemoglobin concentration and ESR level. 15/25 patients had (-174G/G) gene promoter polymorphism, 8/25 were GC and 2/25 were CC. All controls were GG. There was significant association between gene polymorphism and age at disease onset (p = .0172), which was older in those with GG genotype (38.5 ± 10.25 years) than those with CC (33.5 ± 0.71 years) and younger in GC genotypes (27.9 ± 7.9 years). None of the other clinical, laboratory or radiological parameters would predict the IL-6 promoter polymorphism. Conclusion: Serum IL-6 levels and -174G/C promoter polymorphism were higher in RA patients than in healthy controls. The positive correlation of IL-6 level with the DAS28 and duration of morning stiffness may confirm its’ increased involvement in the pathogenesis of RA and may point to the need for considering of anti-IL-6 agents in their management plan. The negative correlation of IL-6 level with the hemoglobin level may confirm IL-6 play a significant role in anemia of RA. Keywords: IL-6, HCV, (-174G/C) Promoter polymorphism, Rheumatoid arthritishttp://www.sciencedirect.com/science/article/pii/S1110863017301209 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Reham Mohamed Raafat Hamed Soliman Aref Mohamed Reham Ali Dwedar Yasmine Samy Elkholy Fatema Talaat Elgengehy |
spellingShingle |
Reham Mohamed Raafat Hamed Soliman Aref Mohamed Reham Ali Dwedar Yasmine Samy Elkholy Fatema Talaat Elgengehy Association of interleukin-6 and its -174G/C promoter polymorphism with clinical and laboratory characteristics of non hepatitis C virus rheumatoid arthritis patients Egyptian Journal of Medical Human Genetics |
author_facet |
Reham Mohamed Raafat Hamed Soliman Aref Mohamed Reham Ali Dwedar Yasmine Samy Elkholy Fatema Talaat Elgengehy |
author_sort |
Reham Mohamed Raafat Hamed |
title |
Association of interleukin-6 and its -174G/C promoter polymorphism with clinical and laboratory characteristics of non hepatitis C virus rheumatoid arthritis patients |
title_short |
Association of interleukin-6 and its -174G/C promoter polymorphism with clinical and laboratory characteristics of non hepatitis C virus rheumatoid arthritis patients |
title_full |
Association of interleukin-6 and its -174G/C promoter polymorphism with clinical and laboratory characteristics of non hepatitis C virus rheumatoid arthritis patients |
title_fullStr |
Association of interleukin-6 and its -174G/C promoter polymorphism with clinical and laboratory characteristics of non hepatitis C virus rheumatoid arthritis patients |
title_full_unstemmed |
Association of interleukin-6 and its -174G/C promoter polymorphism with clinical and laboratory characteristics of non hepatitis C virus rheumatoid arthritis patients |
title_sort |
association of interleukin-6 and its -174g/c promoter polymorphism with clinical and laboratory characteristics of non hepatitis c virus rheumatoid arthritis patients |
publisher |
SpringerOpen |
series |
Egyptian Journal of Medical Human Genetics |
issn |
1110-8630 |
publishDate |
2018-07-01 |
description |
Background: Interleukin-6 is a cytokine protein, which causes inflammation, maintains immune homeostasis and shows a role in rheumatoid arthritis pathogenesis. IL-6-174G/C promoter polymorphism may have a role in susceptibility to RA. Aim of the work: To evaluate the clinical significance of serum levels of IL-6 and its -174G/C promoter polymorphism in RA patients in comparison with the controls. Patients and methods: This study enrolled 25 non hepatitis C virus RA patients versus 25 age and gender matched controls. Demographic, clinical and laboratory data were prospectively evaluated. Serum IL-6 level and promoter (-174G/C) genotype were determined. Results: Serum IL-6 levels was significantly higher in RA patients compared to control subjects (p = .001), especially those with CC promoter polymorphism. There was a significant correlation between IL and 6 level and duration of morning stiffness, disease activity, hemoglobin concentration and ESR level. 15/25 patients had (-174G/G) gene promoter polymorphism, 8/25 were GC and 2/25 were CC. All controls were GG. There was significant association between gene polymorphism and age at disease onset (p = .0172), which was older in those with GG genotype (38.5 ± 10.25 years) than those with CC (33.5 ± 0.71 years) and younger in GC genotypes (27.9 ± 7.9 years). None of the other clinical, laboratory or radiological parameters would predict the IL-6 promoter polymorphism. Conclusion: Serum IL-6 levels and -174G/C promoter polymorphism were higher in RA patients than in healthy controls. The positive correlation of IL-6 level with the DAS28 and duration of morning stiffness may confirm its’ increased involvement in the pathogenesis of RA and may point to the need for considering of anti-IL-6 agents in their management plan. The negative correlation of IL-6 level with the hemoglobin level may confirm IL-6 play a significant role in anemia of RA. Keywords: IL-6, HCV, (-174G/C) Promoter polymorphism, Rheumatoid arthritis |
url |
http://www.sciencedirect.com/science/article/pii/S1110863017301209 |
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