A Newly Identified LncRNA LncIMF4 Controls Adipogenesis of Porcine Intramuscular Preadipocyte through Attenuating Autophagy to Inhibit Lipolysis

A Newly Identified LncRNA LncIMF4 Controls Adipogenesis of Porcine Intramuscular Preadipocyte through Attenuating Autophagy to Inhibit Lipolysis

Intramuscular fat (IMF) is implicated in juiciness, tenderness, and flavor of pork. Meat quality of Chinese fat-type pig is much better than that of lean-type pig because of its higher IMF content. LncRNA is a vital regulator that contributes to adipogenesis. However, it is unknown about the regulat...

Full description

Bibliographic Details
Main Authors: Yunmei Sun, Rui Cai, Yingqian Wang, Rui Zhao, Jin Qin, Weijun Pang
Format: Article
Language:English
Published: MDPI AG 2020-05-01
Series:Animals
Subjects:
pork quality
lncIMF4
intramuscular preadipocyte
differentiation
autophagy
Online Access:https://www.mdpi.com/2076-2615/10/6/926
id doaj-9757eaefb63840798a728d0f7142f7d2
record_format Article
spelling doaj-9757eaefb63840798a728d0f7142f7d22020-11-25T02:21:24ZengMDPI AGAnimals2076-26152020-05-011092692610.3390/ani10060926A Newly Identified LncRNA LncIMF4 Controls Adipogenesis of Porcine Intramuscular Preadipocyte through Attenuating Autophagy to Inhibit LipolysisYunmei Sun0Rui Cai1Yingqian Wang2Rui Zhao3Jin Qin4Weijun Pang5Laboratory of Animal Fat Deposition and Muscle Development, Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, ChinaLaboratory of Animal Fat Deposition and Muscle Development, Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, ChinaLaboratory of Animal Fat Deposition and Muscle Development, Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, ChinaLaboratory of Animal Fat Deposition and Muscle Development, Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, ChinaLaboratory of Animal Fat Deposition and Muscle Development, Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, ChinaLaboratory of Animal Fat Deposition and Muscle Development, Key Laboratory of Animal Genetics, Breeding and Reproduction of Shaanxi Province, College of Animal Science and Technology, Northwest A&F University, Yangling, Shaanxi 712100, ChinaIntramuscular fat (IMF) is implicated in juiciness, tenderness, and flavor of pork. Meat quality of Chinese fat-type pig is much better than that of lean-type pig because of its higher IMF content. LncRNA is a vital regulator that contributes to adipogenesis. However, it is unknown about the regulation of lncRNA on IMF content. Here, by RNA sequence analysis of intramuscular adipocyte from Bamei pig (fat-type) and Yorkshire pig (lean-type), we found that a novel lncRNA, lncIMF4, was associated with adipogenesis. LncIMF4, abundant in adipose, differently expressed along with intramuscular preadipocyte proliferation and differentiation. Meanwhile, it is located both in cytoplasm and nucleus. Besides, lncIMF4 knockdown promoted proliferation and differentiation of porcine intramuscular preadipocytes, whereas inhibited autophagy. Moreover, lncIMF4 knockdown facilitated intramuscular adipogenesis through attenuating autophagy to repress the lipolysis. Our findings will contribute to understand better the mechanism of lncRNA controlling intramuscular adipogenesis for promoting pork quality.https://www.mdpi.com/2076-2615/10/6/926pork qualitylncIMF4intramuscular preadipocytedifferentiationautophagy
collection DOAJ
language English
format Article
sources DOAJ
author Yunmei Sun
Rui Cai
Yingqian Wang
Rui Zhao
Jin Qin
Weijun Pang
spellingShingle Yunmei Sun
Rui Cai
Yingqian Wang
Rui Zhao
Jin Qin
Weijun Pang
A Newly Identified LncRNA LncIMF4 Controls Adipogenesis of Porcine Intramuscular Preadipocyte through Attenuating Autophagy to Inhibit Lipolysis
Animals
pork quality
lncIMF4
intramuscular preadipocyte
differentiation
autophagy
author_facet Yunmei Sun
Rui Cai
Yingqian Wang
Rui Zhao
Jin Qin
Weijun Pang
author_sort Yunmei Sun
title A Newly Identified LncRNA LncIMF4 Controls Adipogenesis of Porcine Intramuscular Preadipocyte through Attenuating Autophagy to Inhibit Lipolysis
title_short A Newly Identified LncRNA LncIMF4 Controls Adipogenesis of Porcine Intramuscular Preadipocyte through Attenuating Autophagy to Inhibit Lipolysis
title_full A Newly Identified LncRNA LncIMF4 Controls Adipogenesis of Porcine Intramuscular Preadipocyte through Attenuating Autophagy to Inhibit Lipolysis
title_fullStr A Newly Identified LncRNA LncIMF4 Controls Adipogenesis of Porcine Intramuscular Preadipocyte through Attenuating Autophagy to Inhibit Lipolysis
title_full_unstemmed A Newly Identified LncRNA LncIMF4 Controls Adipogenesis of Porcine Intramuscular Preadipocyte through Attenuating Autophagy to Inhibit Lipolysis
title_sort newly identified lncrna lncimf4 controls adipogenesis of porcine intramuscular preadipocyte through attenuating autophagy to inhibit lipolysis
publisher MDPI AG
series Animals
issn 2076-2615
publishDate 2020-05-01
description Intramuscular fat (IMF) is implicated in juiciness, tenderness, and flavor of pork. Meat quality of Chinese fat-type pig is much better than that of lean-type pig because of its higher IMF content. LncRNA is a vital regulator that contributes to adipogenesis. However, it is unknown about the regulation of lncRNA on IMF content. Here, by RNA sequence analysis of intramuscular adipocyte from Bamei pig (fat-type) and Yorkshire pig (lean-type), we found that a novel lncRNA, lncIMF4, was associated with adipogenesis. LncIMF4, abundant in adipose, differently expressed along with intramuscular preadipocyte proliferation and differentiation. Meanwhile, it is located both in cytoplasm and nucleus. Besides, lncIMF4 knockdown promoted proliferation and differentiation of porcine intramuscular preadipocytes, whereas inhibited autophagy. Moreover, lncIMF4 knockdown facilitated intramuscular adipogenesis through attenuating autophagy to repress the lipolysis. Our findings will contribute to understand better the mechanism of lncRNA controlling intramuscular adipogenesis for promoting pork quality.
topic pork quality
lncIMF4
intramuscular preadipocyte
differentiation
autophagy
url https://www.mdpi.com/2076-2615/10/6/926
work_keys_str_mv AT yunmeisun anewlyidentifiedlncrnalncimf4controlsadipogenesisofporcineintramuscularpreadipocytethroughattenuatingautophagytoinhibitlipolysis
AT ruicai anewlyidentifiedlncrnalncimf4controlsadipogenesisofporcineintramuscularpreadipocytethroughattenuatingautophagytoinhibitlipolysis
AT yingqianwang anewlyidentifiedlncrnalncimf4controlsadipogenesisofporcineintramuscularpreadipocytethroughattenuatingautophagytoinhibitlipolysis
AT ruizhao anewlyidentifiedlncrnalncimf4controlsadipogenesisofporcineintramuscularpreadipocytethroughattenuatingautophagytoinhibitlipolysis
AT jinqin anewlyidentifiedlncrnalncimf4controlsadipogenesisofporcineintramuscularpreadipocytethroughattenuatingautophagytoinhibitlipolysis
AT weijunpang anewlyidentifiedlncrnalncimf4controlsadipogenesisofporcineintramuscularpreadipocytethroughattenuatingautophagytoinhibitlipolysis
AT yunmeisun newlyidentifiedlncrnalncimf4controlsadipogenesisofporcineintramuscularpreadipocytethroughattenuatingautophagytoinhibitlipolysis
AT ruicai newlyidentifiedlncrnalncimf4controlsadipogenesisofporcineintramuscularpreadipocytethroughattenuatingautophagytoinhibitlipolysis
AT yingqianwang newlyidentifiedlncrnalncimf4controlsadipogenesisofporcineintramuscularpreadipocytethroughattenuatingautophagytoinhibitlipolysis
AT ruizhao newlyidentifiedlncrnalncimf4controlsadipogenesisofporcineintramuscularpreadipocytethroughattenuatingautophagytoinhibitlipolysis
AT jinqin newlyidentifiedlncrnalncimf4controlsadipogenesisofporcineintramuscularpreadipocytethroughattenuatingautophagytoinhibitlipolysis
AT weijunpang newlyidentifiedlncrnalncimf4controlsadipogenesisofporcineintramuscularpreadipocytethroughattenuatingautophagytoinhibitlipolysis
_version_ 1724866422652272640

Similar Items