Preliminary study on the anti-inflammatory effect of magnolol on LPSinduced mice

• Main Authors: Si-Yu Mo, Lin-Quan Zang
• Format: Article
• Language: English
• Published: Editorial Board of Journal of Hainan Medical University 2019-03-01
• Series: Journal of Hainan Medical University
• Online Access: http://www.hnykdxxb.com/PDF/201905/04.pdf

Objective: Investigating the anti-inflammatory effects of magnolol on lipopolysaccharide (LPS)-induced inflammation model mouse and its effect on NF-κB pathway. Methods: Mice were randomly divided into 5 group: normal control group, inflammatory model group, dexamethasone positive drug group and, magnolol group. After administrating for 7 d, LPS was intraperitoneally injected to induce inflammatory on the 8th day, and blood was taken 3 h later and the thymus and spleen were weighed. The levels of serum TNF-α, IL-22 and IL- 17 were detected by ELISA method. The expression levels of TNF-α, NF-κB p65 and IL- 17 in thymus were detected by immunohistochemistry. Results: Magnolol could evidently reduce the levels of serum TNF-α, NF-κB p65 and IL-17 and IL-22. Immunohistochemistry result showed that magnolol could down-regulate the expression of IL-17, TNF-α and p65. Conclusion: Magnolol can prevent and treat LPS-induced inflammation. Its anti-inflammatory effect is connected with the down-regulation of TNF-α and NF-κB p65 protein levels by IL- 17 inflammatory pathway. This experiment provides a theoretical basis for magnolol in antiinflammatory.