Preliminary study on the anti-inflammatory effect of magnolol on LPSinduced mice
Objective: Investigating the anti-inflammatory effects of magnolol on lipopolysaccharide (LPS)-induced inflammation model mouse and its effect on NF-κB pathway. Methods: Mice were randomly divided into 5 group: normal control group, inflammatory model group, dexamethasone positive drug group and,...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Editorial Board of Journal of Hainan Medical University
2019-03-01
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| Series: | Journal of Hainan Medical University |
| Online Access: | http://www.hnykdxxb.com/PDF/201905/04.pdf |
| Summary: | Objective: Investigating the anti-inflammatory effects of magnolol on lipopolysaccharide
(LPS)-induced inflammation model mouse and its effect on NF-κB pathway. Methods:
Mice were randomly divided into 5 group: normal control group, inflammatory model group,
dexamethasone positive drug group and, magnolol group. After administrating for 7 d, LPS
was intraperitoneally injected to induce inflammatory on the 8th day, and blood was taken 3
h later and the thymus and spleen were weighed. The levels of serum TNF-α, IL-22 and IL-
17 were detected by ELISA method. The expression levels of TNF-α, NF-κB p65 and IL-
17 in thymus were detected by immunohistochemistry. Results: Magnolol could evidently
reduce the levels of serum TNF-α, NF-κB p65 and IL-17 and IL-22. Immunohistochemistry
result showed that magnolol could down-regulate the expression of IL-17, TNF-α and p65.
Conclusion: Magnolol can prevent and treat LPS-induced inflammation. Its anti-inflammatory
effect is connected with the down-regulation of TNF-α and NF-κB p65 protein levels by IL-
17 inflammatory pathway. This experiment provides a theoretical basis for magnolol in antiinflammatory. |
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| ISSN: | 1007-1237 1007-1237 |