Dependence of DNA-Histograms on The Sampling Techniques in Fine Needle Aspirates of the Breast

Dependence of DNA-Histograms on The Sampling Techniques in Fine Needle Aspirates of the Breast

48 fine needle aspiration biopsy (FNAB) samples from 25 breast cancer cases, originally used for cytodiagnosis were subjected to DNA cytometry. There were air dried smears stained with the MGG method, and samples stained with HE or PAP stain after 50% ethanol fixation and cytocentrifugation. Differe...

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Main Authors: A. Elzagheid, Y. Collan
Format: Article
Language:English
Published: Hindawi Limited 2002-01-01
Series:Analytical Cellular Pathology
Online Access:http://dx.doi.org/10.1155/2002/393686
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spelling doaj-970283e29cf84b0f8e8c758c2f7775d92020-11-24T23:44:15ZengHindawi LimitedAnalytical Cellular Pathology0921-89121878-36512002-01-01244-515916510.1155/2002/393686Dependence of DNA-Histograms on The Sampling Techniques in Fine Needle Aspirates of the BreastA. Elzagheid0Y. Collan1Department of Pathology, University of Turku, Kiinamyllynkatu 10, FIN‐20520, Turku, FinlandDepartment of Pathology, University of Turku, Kiinamyllynkatu 10, FIN‐20520, Turku, Finland48 fine needle aspiration biopsy (FNAB) samples from 25 breast cancer cases, originally used for cytodiagnosis were subjected to DNA cytometry. There were air dried smears stained with the MGG method, and samples stained with HE or PAP stain after 50% ethanol fixation and cytocentrifugation. Different sampling strategies were applied. Four methods were tested: method 1: cell groups measured, method 2: all cells measured, method 3: free cells measured, and method 4: atypical free cells measured. Method 4 showed most often DNA aneuploid histogram patterns, sampling method 1 had the highest number of DNA diploid histogram patterns. Diagnostic approaches may benefit from a sampling method detecting the hiding aneuploid cell population. Grading of neoplasm could potentially benefit from other approaches.http://dx.doi.org/10.1155/2002/393686
collection DOAJ
language English
format Article
sources DOAJ
author A. Elzagheid
Y. Collan
spellingShingle A. Elzagheid
Y. Collan
Dependence of DNA-Histograms on The Sampling Techniques in Fine Needle Aspirates of the Breast
Analytical Cellular Pathology
author_facet A. Elzagheid
Y. Collan
author_sort A. Elzagheid
title Dependence of DNA-Histograms on The Sampling Techniques in Fine Needle Aspirates of the Breast
title_short Dependence of DNA-Histograms on The Sampling Techniques in Fine Needle Aspirates of the Breast
title_full Dependence of DNA-Histograms on The Sampling Techniques in Fine Needle Aspirates of the Breast
title_fullStr Dependence of DNA-Histograms on The Sampling Techniques in Fine Needle Aspirates of the Breast
title_full_unstemmed Dependence of DNA-Histograms on The Sampling Techniques in Fine Needle Aspirates of the Breast
title_sort dependence of dna-histograms on the sampling techniques in fine needle aspirates of the breast
publisher Hindawi Limited
series Analytical Cellular Pathology
issn 0921-8912
1878-3651
publishDate 2002-01-01
description 48 fine needle aspiration biopsy (FNAB) samples from 25 breast cancer cases, originally used for cytodiagnosis were subjected to DNA cytometry. There were air dried smears stained with the MGG method, and samples stained with HE or PAP stain after 50% ethanol fixation and cytocentrifugation. Different sampling strategies were applied. Four methods were tested: method 1: cell groups measured, method 2: all cells measured, method 3: free cells measured, and method 4: atypical free cells measured. Method 4 showed most often DNA aneuploid histogram patterns, sampling method 1 had the highest number of DNA diploid histogram patterns. Diagnostic approaches may benefit from a sampling method detecting the hiding aneuploid cell population. Grading of neoplasm could potentially benefit from other approaches.
url http://dx.doi.org/10.1155/2002/393686
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AT ycollan dependenceofdnahistogramsonthesamplingtechniquesinfineneedleaspiratesofthebreast
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