Production of taxadiene from cultured ginseng roots transformed with taxadiene synthase gene

Production of taxadiene from cultured ginseng roots transformed with taxadiene synthase gene

Paclitaxel is produced by various species of yew trees and hasbeen extensively used to treat tumors. In our research, ataxadiene synthase (TS) gene from Taxus brevifolia was used totransform the roots of cultured ginseng (Panax ginseng C.A.Meyer) to produce taxadiene, the unique skeletal precursor t...

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Main Author: Mijeong Cha1, Sang Hee Shim1, Sung Hong Kim2, Ok Tae Kim3, Se-Weon Lee4, Suk-Yoon Kwon5 & Kwang-Hyun Baek1,*
Format: Article
Language:English
Published: Korean Society for Biochemistry and Molecular Biology 2012-10-01
Series:BMB Reports
Subjects:
Panax ginseng C.A. Meyer
Root culture
Taxadiene synthase
Taxol
Transformation
Online Access:http://www.jbmb.or.kr/jbmb/pdf.php?data=MTMwMTIyMTdAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0NS0xMCU1RDEyMTAyOTIxMjVfJTI4NTg5LTU5NCUyOUJNQl8xMi0wODUucGRm
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spelling doaj-96fe1cbe4cea43409b74fd2f0dc6e0112020-11-24T23:46:07ZengKorean Society for Biochemistry and Molecular BiologyBMB Reports1976-66961976-670X2012-10-014510589594Production of taxadiene from cultured ginseng roots transformed with taxadiene synthase gene Mijeong Cha1, Sang Hee Shim1, Sung Hong Kim2, Ok Tae Kim3, Se-Weon Lee4, Suk-Yoon Kwon5 & Kwang-Hyun Baek1,*Paclitaxel is produced by various species of yew trees and hasbeen extensively used to treat tumors. In our research, ataxadiene synthase (TS) gene from Taxus brevifolia was used totransform the roots of cultured ginseng (Panax ginseng C.A.Meyer) to produce taxadiene, the unique skeletal precursor totaxol. The TS gene was successfully introduced into theginseng genome, and the de novo formation of taxadiene wasidentified by mass spectroscopy profiling. Without any changein phenotypes or growth difference in a TS-transgenic ginsengline, the transgenic TSS3-2 line accumulated 9.1 μg taxadieneper gram of dry weight. In response to the treatment of methyljasmonate for 3 or 6 days, the accumulation was 14.6 and15.9 μg per g of dry weight, respectively. This is the first reportof the production of taxadiene by engineering ginseng rootswith a taxadiene synthase gene. http://www.jbmb.or.kr/jbmb/pdf.php?data=MTMwMTIyMTdAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0NS0xMCU1RDEyMTAyOTIxMjVfJTI4NTg5LTU5NCUyOUJNQl8xMi0wODUucGRmPanax ginseng C.A. MeyerRoot cultureTaxadiene synthaseTaxolTransformation
collection DOAJ
language English
format Article
sources DOAJ
author Mijeong Cha1, Sang Hee Shim1, Sung Hong Kim2, Ok Tae Kim3, Se-Weon Lee4, Suk-Yoon Kwon5 & Kwang-Hyun Baek1,*
spellingShingle Mijeong Cha1, Sang Hee Shim1, Sung Hong Kim2, Ok Tae Kim3, Se-Weon Lee4, Suk-Yoon Kwon5 & Kwang-Hyun Baek1,*
Production of taxadiene from cultured ginseng roots transformed with taxadiene synthase gene
BMB Reports
Panax ginseng C.A. Meyer
Root culture
Taxadiene synthase
Taxol
Transformation
author_facet Mijeong Cha1, Sang Hee Shim1, Sung Hong Kim2, Ok Tae Kim3, Se-Weon Lee4, Suk-Yoon Kwon5 & Kwang-Hyun Baek1,*
author_sort Mijeong Cha1, Sang Hee Shim1, Sung Hong Kim2, Ok Tae Kim3, Se-Weon Lee4, Suk-Yoon Kwon5 & Kwang-Hyun Baek1,*
title Production of taxadiene from cultured ginseng roots transformed with taxadiene synthase gene
title_short Production of taxadiene from cultured ginseng roots transformed with taxadiene synthase gene
title_full Production of taxadiene from cultured ginseng roots transformed with taxadiene synthase gene
title_fullStr Production of taxadiene from cultured ginseng roots transformed with taxadiene synthase gene
title_full_unstemmed Production of taxadiene from cultured ginseng roots transformed with taxadiene synthase gene
title_sort production of taxadiene from cultured ginseng roots transformed with taxadiene synthase gene
publisher Korean Society for Biochemistry and Molecular Biology
series BMB Reports
issn 1976-6696
1976-670X
publishDate 2012-10-01
description Paclitaxel is produced by various species of yew trees and hasbeen extensively used to treat tumors. In our research, ataxadiene synthase (TS) gene from Taxus brevifolia was used totransform the roots of cultured ginseng (Panax ginseng C.A.Meyer) to produce taxadiene, the unique skeletal precursor totaxol. The TS gene was successfully introduced into theginseng genome, and the de novo formation of taxadiene wasidentified by mass spectroscopy profiling. Without any changein phenotypes or growth difference in a TS-transgenic ginsengline, the transgenic TSS3-2 line accumulated 9.1 μg taxadieneper gram of dry weight. In response to the treatment of methyljasmonate for 3 or 6 days, the accumulation was 14.6 and15.9 μg per g of dry weight, respectively. This is the first reportof the production of taxadiene by engineering ginseng rootswith a taxadiene synthase gene.
topic Panax ginseng C.A. Meyer
Root culture
Taxadiene synthase
Taxol
Transformation
url http://www.jbmb.or.kr/jbmb/pdf.php?data=MTMwMTIyMTdAcGRmX3JhaW50cmFjZV9sZWV5c0AlNUI0NS0xMCU1RDEyMTAyOTIxMjVfJTI4NTg5LTU5NCUyOUJNQl8xMi0wODUucGRm
work_keys_str_mv AT mijeongcha1sangheeshim1sunghongkim2oktaekim3seweonlee4sukyoonkwon5kwanghyunbaek1 productionoftaxadienefromculturedginsengrootstransformedwithtaxadienesynthasegene
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