Summary: | The
influence of low-intensity electromagnetic radiation (EMR) on the content of
phenolic compounds (PC) in plants on different stages of ontogeny is discussed.
A medicinal plant (Calendula officinalis L.) and agricultural crop Fagopyrum
sagittatum G., diploid varieties and tetraploid varieties were chosen as objects of study. Microwave
EMR in two frequency bands, 53.57-78.33 GHz (wide range mode) and 64.00-66.00
GHz (narrow range mode), has been selected to learn the physical effects on
seeds, with a treatment exposure time of 20, 12, or 8 min. The experiments were
conducted under laboratory and field conditions. Treated and control seeds were
germinated by 100 pcs in a growth chamber on moistened filter paper at 22–24˚C
in the dark and placed on the daylight from the third day. The level of PC in
the seedlings was measured with the Folin-Ciocalteu reagent. It was found that
in the EMR pretreated C. officinalis seedlings the level of PC is maximal on
14th day of germination. The wide mode treatment for 12 min results in a
two-fold increase in the level of PC in juvenile shoots with respect to
control. Different constitutional levels of phenolic compounds in the leaves of
diploid and tetraploid varieties of F. sagittatum in the most critical phases
of ontogeny were found. It is recommended to use EMR with a 12 min exposure
time for C. officinalis. The narrow frequency mode with an exposure time of 20 min
is preferable to use for most varieties of F. sagittatum.
|