THE PECULIARITIES OF THE LIPASES BIOSYNTHESIS BY ASPERGILLUS NIGER CNMN FD 01L STRAIN ON THE MEDIA WITH OPTIMAL COMPOSITION

• Main Authors: О. А. Десятник, Ж. П. Тюрина, С. В. Лаблюк, О. А. Болога, А. Г. Лазареску
• Format: Article
• Language: English
• Published: Odessa I. I. Mechnikov National University 2007-07-01
• Series: Mìkrobìologìâ ì Bìotehnologìâ
• Subjects: ліпази; aspergillus niger; комплексні сполуки кобальта і заліза; стимулятори біосинтезу
• Online Access: http://mbt.onu.edu.ua/article/view/107773

The article contains date referring to the using of coordinative compounds of microelements for the regulation of the biosynthesis of microbial lipases. As the object of biological investigations Aspergillus niger CNMN FD 01L strain, selected as perspective producer of exocellular enzymes with lipolytic action was used. Aspergillus niger CNMN FD 01L strain synthesize unspecific lipases, which hydrolyse all the ester binding of triglyceride independent of their location. For increasing of enzymatic activity of the strain there were studied about 60 coordinative compounds of Co, Fe, Ni, Cu, Zn and other, containing in ligands composition pyridine, aniline, thiocarbamid, metil-and dimetilglioxime, amino acids (methionin, glicin, valin) etc. In the result on the first screening three substances: [Co(MgH)2Py2]BF4H2O, CoDigsemiCl24H2O, FeO3•Mеt have been selected as possible stimulators of the lipases biosynthesis by Aspergillus niger CNMN FD 01L strain. Maximum effect was registered in the variant with the coordinative compound: [Co(MgH)2Py2]BF4H2O in concentration 15 mg/l. Also, it was established the using of coordinative compounds has not influenced the maximum of lipases accumulation, which is shown at the 5th day of producer cultivation on the control medium (without coordinative compound) as well as on the optimized medium, being 12558 u/ml and 17731 u/ml, respectively.