Wnt-1 expression in PC12 cells induces exon 15 deletion and expression of L-APP

• Main Authors: Peter J Morin, Miguel Medina, Mikhail Semenov, Anthony M.C Brown, Kenneth S Kosik
• Format: Article
• Language: English
• Published: Elsevier 2004-06-01
• Series: Neurobiology of Disease
• Subjects: Wnt-1; APP isoform; Alzheimer's disease
• Online Access: http://www.sciencedirect.com/science/article/pii/S0969996104000105
• ISSN: 1095-953X

The metabolism of amyloid precursor protein (APP) is central to Alzheimer's disease pathogenesis. Recent data have linked APP and presenilin to the Wnt/wingless signaling pathway. To assess affects of Wnt stimulation on APP isoform expression, we infected PC12 cells and C57MG cells with a retrovirus containing murine Wnt-1. In PC12 cells, Wnt-1 expression is associated with induction of exon 15 deletion from APP mRNA and expression of L-APP. Our data suggest that APP isoform expression is regulated, in part, by the Wnt/wingless signaling pathway.