Identification and determination of antibiotic resistance of pathogenic bacteria Isolated from Septic Wounds

• Main Authors: Alsadig Mohammed Abdalla, Abdelkader Alsanousi G. Elzen, Ahmed Alshahed, Gurfa Abu Azoom Sh. Az., Aziza Heeba M.O., Gaeidaa A. Mohammed M.A., Habsa Yunis Ab. K., Nisreen Mohammed Ab
• Format: Article
• Language: English
• Published: Society of Open Science 2015-10-01
• Series: Journal of Advanced Laboratory Research in Biology
• Subjects: Septic wounds; Pathogenic Bacteria; Identification and Determination of Antibiotic Resistance
• Online Access: https://e-journal.sospublication.co.in/index.php/jalrb/article/view/234

Wound infection is a global cause of morbidity and mortality across all wound types. Therefore, efficient diagnosis and treatment of wound infection are essential. This study was carried out to identify the pathogenic bacteria in infected wounds of the patient’s attending Sebha city hospitals (Libya) and to determine their resistance profile to the most common antibiotics used in therapy. A total of sixty wound swab specimens were collected and cultured, of which 39 samples showed bacterial growth. Three different species of bacteria were isolated. Staphylococcus aureus 21 (53.9%) were the most common organisms followed by Pseudomonas aeruginosa 10 (25.6%) and Staphylococcus epidermidis 8 (20.5%). The antibiotic susceptibility test of the bacterial isolate was performed by Kirby-Bauer disk diffusion method. Results showed that 90.5% of the Staphylococcus aureus isolates were resistant to vancomycin, 61.9% to tetracycline, 57.1% to amoxicillin, 52.4 % to methicillin, 42.9 to erythromycin and 23.8% to streptomycin. 87.5% of the Staphylococcus epidermidis isolates were resistant to vancomycin, 75% to methicillin, 62.5% to tetracycline, 50% to streptomycin 37.5% to amoxicillin, and erythromycin. All the Pseudomonas aeruginosa isolates were sensitive to ciprofloxacin and highly resistant 90-100% to other antibiotics tested Amoxicillin, Nalidixic acid, Streptomycin, and Tetracycline. The high rate of multiple antibiotic resistance was observed in all bacterial species recovered.