Protective effect of kaempferol, a flavonoid widely present in varieties of edible plants, on IL-1β-induced inflammatory response via inhibiting MAPK, Akt, and NF-κB signalling in SW982 cells

Protective effect of kaempferol, a flavonoid widely present in varieties of edible plants, on IL-1β-induced inflammatory response via inhibiting MAPK, Akt, and NF-κB signalling in SW982 cells

Effects and mechanisms of kaempferol, identified in a wide variety of dietary sources, on the expression of interleukin (IL)-6, IL-8, matrix metallo proteinase (MMP)-1, and cyclooxygenase (COX)-2 were evaluated in IL-1β-induced SW982 cells. The cells were pretreated with kaempferol of various concen...

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Main Authors: Jun-Jiang Lian, Bin-Feng Cheng, Yao-Xin Gao, Han Xue, Lei Wang, Mian Wang, Hai-Jie Yang, Zhi-Wei Feng
Format: Article
Language:English
Published: Elsevier 2016-12-01
Series:Journal of Functional Foods
Subjects:
Kaempferol
Inflammatory mediator
Signalling pathway
SW982 cells
Online Access:http://www.sciencedirect.com/science/article/pii/S1756464616302791
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spelling doaj-96a36f07fce14b7eb92c662e7a677e272021-04-30T07:08:46ZengElsevierJournal of Functional Foods1756-46462016-12-0127214222Protective effect of kaempferol, a flavonoid widely present in varieties of edible plants, on IL-1β-induced inflammatory response via inhibiting MAPK, Akt, and NF-κB signalling in SW982 cellsJun-Jiang Lian0Bin-Feng Cheng1Yao-Xin Gao2Han Xue3Lei Wang4Mian Wang5Hai-Jie Yang6Zhi-Wei Feng7College of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, Henan, ChinaCorresponding authors. College of Life Science and Technology, Xinxiang Medical University, 601 Jinsui Road, Xinxiang 453003, Henan, China. Fax: +86 373 3029887.; College of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, Henan, ChinaCollege of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, Henan, ChinaCollege of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, Henan, ChinaCollege of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, Henan, ChinaCollege of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, Henan, ChinaCollege of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, Henan, ChinaCorresponding authors. College of Life Science and Technology, Xinxiang Medical University, 601 Jinsui Road, Xinxiang 453003, Henan, China. Fax: +86 373 3029887.; College of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, Henan, ChinaEffects and mechanisms of kaempferol, identified in a wide variety of dietary sources, on the expression of interleukin (IL)-6, IL-8, matrix metallo proteinase (MMP)-1, and cyclooxygenase (COX)-2 were evaluated in IL-1β-induced SW982 cells. The cells were pretreated with kaempferol of various concentrations (2, 10 and 50 µmol/L) followed by IL-1β (10 ng/mL) stimulation. Kaempferol significantly suppressed mRNA and protein levels of IL-6, IL-8, MMP-1, and COX-2 induced by IL-1β. Kaempferol also inhibited mitogen-activated protein kinase (MAPK) and protein kinase B (Akt) phosphorylation and decreased IκB-α degradation and p65 protein translocation into the nucleus. Moreover, adenosine monophosphate-activated protein kinase (AMPK) does not contribute to the anti-inflammatory effects in SW982 cells, although its phosphorylation is significantly increased by kaempferol. These results indicate that kaempferol may have an anti-inflammatory effect by inhibiting expression of IL-6, IL-8, MMP-1 and COX-2 via suppressing MAPK, Akt, and nuclear factor (NF)-κB signalling but not activating AMPK signalling.http://www.sciencedirect.com/science/article/pii/S1756464616302791KaempferolInflammatory mediatorSignalling pathwaySW982 cells
collection DOAJ
language English
format Article
sources DOAJ
author Jun-Jiang Lian
Bin-Feng Cheng
Yao-Xin Gao
Han Xue
Lei Wang
Mian Wang
Hai-Jie Yang
Zhi-Wei Feng
spellingShingle Jun-Jiang Lian
Bin-Feng Cheng
Yao-Xin Gao
Han Xue
Lei Wang
Mian Wang
Hai-Jie Yang
Zhi-Wei Feng
Protective effect of kaempferol, a flavonoid widely present in varieties of edible plants, on IL-1β-induced inflammatory response via inhibiting MAPK, Akt, and NF-κB signalling in SW982 cells
Journal of Functional Foods
Kaempferol
Inflammatory mediator
Signalling pathway
SW982 cells
author_facet Jun-Jiang Lian
Bin-Feng Cheng
Yao-Xin Gao
Han Xue
Lei Wang
Mian Wang
Hai-Jie Yang
Zhi-Wei Feng
author_sort Jun-Jiang Lian
title Protective effect of kaempferol, a flavonoid widely present in varieties of edible plants, on IL-1β-induced inflammatory response via inhibiting MAPK, Akt, and NF-κB signalling in SW982 cells
title_short Protective effect of kaempferol, a flavonoid widely present in varieties of edible plants, on IL-1β-induced inflammatory response via inhibiting MAPK, Akt, and NF-κB signalling in SW982 cells
title_full Protective effect of kaempferol, a flavonoid widely present in varieties of edible plants, on IL-1β-induced inflammatory response via inhibiting MAPK, Akt, and NF-κB signalling in SW982 cells
title_fullStr Protective effect of kaempferol, a flavonoid widely present in varieties of edible plants, on IL-1β-induced inflammatory response via inhibiting MAPK, Akt, and NF-κB signalling in SW982 cells
title_full_unstemmed Protective effect of kaempferol, a flavonoid widely present in varieties of edible plants, on IL-1β-induced inflammatory response via inhibiting MAPK, Akt, and NF-κB signalling in SW982 cells
title_sort protective effect of kaempferol, a flavonoid widely present in varieties of edible plants, on il-1β-induced inflammatory response via inhibiting mapk, akt, and nf-κb signalling in sw982 cells
publisher Elsevier
series Journal of Functional Foods
issn 1756-4646
publishDate 2016-12-01
description Effects and mechanisms of kaempferol, identified in a wide variety of dietary sources, on the expression of interleukin (IL)-6, IL-8, matrix metallo proteinase (MMP)-1, and cyclooxygenase (COX)-2 were evaluated in IL-1β-induced SW982 cells. The cells were pretreated with kaempferol of various concentrations (2, 10 and 50 µmol/L) followed by IL-1β (10 ng/mL) stimulation. Kaempferol significantly suppressed mRNA and protein levels of IL-6, IL-8, MMP-1, and COX-2 induced by IL-1β. Kaempferol also inhibited mitogen-activated protein kinase (MAPK) and protein kinase B (Akt) phosphorylation and decreased IκB-α degradation and p65 protein translocation into the nucleus. Moreover, adenosine monophosphate-activated protein kinase (AMPK) does not contribute to the anti-inflammatory effects in SW982 cells, although its phosphorylation is significantly increased by kaempferol. These results indicate that kaempferol may have an anti-inflammatory effect by inhibiting expression of IL-6, IL-8, MMP-1 and COX-2 via suppressing MAPK, Akt, and nuclear factor (NF)-κB signalling but not activating AMPK signalling.
topic Kaempferol
Inflammatory mediator
Signalling pathway
SW982 cells
url http://www.sciencedirect.com/science/article/pii/S1756464616302791
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