Improved Salt Tolerance and Metabolomics Analysis of Synechococcus elongatus UTEX 2973 by Overexpressing Mrp Antiporters

Improved Salt Tolerance and Metabolomics Analysis of Synechococcus elongatus UTEX 2973 by Overexpressing Mrp Antiporters

The fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 (Syn2973) is a promising candidate for photosynthetic microbial factory. Seawater utilization is necessary for large-scale cultivation of Syn2973 in the future. However, Syn2973 is sensitive to salt stress, making it necessary to impr...

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Main Authors: Jinyu Cui, Tao Sun, Shubin Li, Yaru Xie, Xinyu Song, Fangzhong Wang, Lei Chen, Weiwen Zhang
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-05-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
cyanobacteria
Mrp antiporter
metabolomic analysis
salt tolerance
tolerance engineering
Online Access:https://www.frontiersin.org/article/10.3389/fbioe.2020.00500/full
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spelling doaj-9674fa143a414adbb5be996de7c0a4f32020-11-25T02:21:25ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852020-05-01810.3389/fbioe.2020.00500521240Improved Salt Tolerance and Metabolomics Analysis of Synechococcus elongatus UTEX 2973 by Overexpressing Mrp AntiportersJinyu Cui0Jinyu Cui1Jinyu Cui2Tao Sun3Tao Sun4Shubin Li5Shubin Li6Shubin Li7Yaru Xie8Yaru Xie9Yaru Xie10Xinyu Song11Xinyu Song12Fangzhong Wang13Fangzhong Wang14Lei Chen15Lei Chen16Lei Chen17Weiwen Zhang18Weiwen Zhang19Weiwen Zhang20Weiwen Zhang21Laboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, ChinaKey Laboratory of Systems Bioengineering, Frontier Science Center for Synthetic Biology, Ministry of Education of China, Tianjin, ChinaCollaborative Innovation Center of Chemical Science and Engineering, Tianjin, ChinaLaboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, ChinaCenter for Biosafety Research and Strategy, Tianjin University, Tianjin, ChinaLaboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, ChinaKey Laboratory of Systems Bioengineering, Frontier Science Center for Synthetic Biology, Ministry of Education of China, Tianjin, ChinaCollaborative Innovation Center of Chemical Science and Engineering, Tianjin, ChinaLaboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, ChinaKey Laboratory of Systems Bioengineering, Frontier Science Center for Synthetic Biology, Ministry of Education of China, Tianjin, ChinaCollaborative Innovation Center of Chemical Science and Engineering, Tianjin, ChinaLaboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, ChinaCenter for Biosafety Research and Strategy, Tianjin University, Tianjin, ChinaLaboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, ChinaCenter for Biosafety Research and Strategy, Tianjin University, Tianjin, ChinaLaboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, ChinaKey Laboratory of Systems Bioengineering, Frontier Science Center for Synthetic Biology, Ministry of Education of China, Tianjin, ChinaCollaborative Innovation Center of Chemical Science and Engineering, Tianjin, ChinaLaboratory of Synthetic Microbiology, School of Chemical Engineering and Technology, Tianjin University, Tianjin, ChinaKey Laboratory of Systems Bioengineering, Frontier Science Center for Synthetic Biology, Ministry of Education of China, Tianjin, ChinaCollaborative Innovation Center of Chemical Science and Engineering, Tianjin, ChinaCenter for Biosafety Research and Strategy, Tianjin University, Tianjin, ChinaThe fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 (Syn2973) is a promising candidate for photosynthetic microbial factory. Seawater utilization is necessary for large-scale cultivation of Syn2973 in the future. However, Syn2973 is sensitive to salt stress, making it necessary to improve its salt tolerance. In this study, 21 exogenous putative transporters were individually overexpressed in Syn2973 to evaluate their effects on salt tolerance. The results showed the overexpression of three Mrp antiporters significantly improved the salt tolerance of Syn2973. Notably, overexpressing the Mrp antiporter from Synechococcus sp. PCC 7002 improved cell growth by 57.7% under 0.4 M NaCl condition. In addition, the metabolomics and biomass composition analyses revealed the possible mechanisms against salt stress in both Syn2973 and the genetically engineered strain. The study provides important engineering strategies to improve salt tolerance of Syn2973 and is valuable for understanding mechanisms of salt tolerance in cyanobacteria.https://www.frontiersin.org/article/10.3389/fbioe.2020.00500/fullcyanobacteriaMrp antiportermetabolomic analysissalt tolerancetolerance engineering
collection DOAJ
language English
format Article
sources DOAJ
author Jinyu Cui
Jinyu Cui
Jinyu Cui
Tao Sun
Tao Sun
Shubin Li
Shubin Li
Shubin Li
Yaru Xie
Yaru Xie
Yaru Xie
Xinyu Song
Xinyu Song
Fangzhong Wang
Fangzhong Wang
Lei Chen
Lei Chen
Lei Chen
Weiwen Zhang
Weiwen Zhang
Weiwen Zhang
Weiwen Zhang
spellingShingle Jinyu Cui
Jinyu Cui
Jinyu Cui
Tao Sun
Tao Sun
Shubin Li
Shubin Li
Shubin Li
Yaru Xie
Yaru Xie
Yaru Xie
Xinyu Song
Xinyu Song
Fangzhong Wang
Fangzhong Wang
Lei Chen
Lei Chen
Lei Chen
Weiwen Zhang
Weiwen Zhang
Weiwen Zhang
Weiwen Zhang
Improved Salt Tolerance and Metabolomics Analysis of Synechococcus elongatus UTEX 2973 by Overexpressing Mrp Antiporters
Frontiers in Bioengineering and Biotechnology
cyanobacteria
Mrp antiporter
metabolomic analysis
salt tolerance
tolerance engineering
author_facet Jinyu Cui
Jinyu Cui
Jinyu Cui
Tao Sun
Tao Sun
Shubin Li
Shubin Li
Shubin Li
Yaru Xie
Yaru Xie
Yaru Xie
Xinyu Song
Xinyu Song
Fangzhong Wang
Fangzhong Wang
Lei Chen
Lei Chen
Lei Chen
Weiwen Zhang
Weiwen Zhang
Weiwen Zhang
Weiwen Zhang
author_sort Jinyu Cui
title Improved Salt Tolerance and Metabolomics Analysis of Synechococcus elongatus UTEX 2973 by Overexpressing Mrp Antiporters
title_short Improved Salt Tolerance and Metabolomics Analysis of Synechococcus elongatus UTEX 2973 by Overexpressing Mrp Antiporters
title_full Improved Salt Tolerance and Metabolomics Analysis of Synechococcus elongatus UTEX 2973 by Overexpressing Mrp Antiporters
title_fullStr Improved Salt Tolerance and Metabolomics Analysis of Synechococcus elongatus UTEX 2973 by Overexpressing Mrp Antiporters
title_full_unstemmed Improved Salt Tolerance and Metabolomics Analysis of Synechococcus elongatus UTEX 2973 by Overexpressing Mrp Antiporters
title_sort improved salt tolerance and metabolomics analysis of synechococcus elongatus utex 2973 by overexpressing mrp antiporters
publisher Frontiers Media S.A.
series Frontiers in Bioengineering and Biotechnology
issn 2296-4185
publishDate 2020-05-01
description The fast-growing cyanobacterium Synechococcus elongatus UTEX 2973 (Syn2973) is a promising candidate for photosynthetic microbial factory. Seawater utilization is necessary for large-scale cultivation of Syn2973 in the future. However, Syn2973 is sensitive to salt stress, making it necessary to improve its salt tolerance. In this study, 21 exogenous putative transporters were individually overexpressed in Syn2973 to evaluate their effects on salt tolerance. The results showed the overexpression of three Mrp antiporters significantly improved the salt tolerance of Syn2973. Notably, overexpressing the Mrp antiporter from Synechococcus sp. PCC 7002 improved cell growth by 57.7% under 0.4 M NaCl condition. In addition, the metabolomics and biomass composition analyses revealed the possible mechanisms against salt stress in both Syn2973 and the genetically engineered strain. The study provides important engineering strategies to improve salt tolerance of Syn2973 and is valuable for understanding mechanisms of salt tolerance in cyanobacteria.
topic cyanobacteria
Mrp antiporter
metabolomic analysis
salt tolerance
tolerance engineering
url https://www.frontiersin.org/article/10.3389/fbioe.2020.00500/full
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