Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems

Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems

Few approaches for targeted manipulation of the epigenome are available in plants. Here, the authors adapt the dCas9-SunTag system to engineer targeted gene activation and site-specific manipulation of DNA methylation in Arabidopsis.

Bibliographic Details
Main Authors: Ashot Papikian, Wanlu Liu, Javier Gallego-Bartolomé, Steven E. Jacobsen
Format: Article
Language:English
Published: Nature Publishing Group 2019-02-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-019-08736-7
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spelling doaj-966d08acb09448ee9fe2347f8253f3632021-05-11T12:38:57ZengNature Publishing GroupNature Communications2041-17232019-02-0110111110.1038/s41467-019-08736-7Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systemsAshot Papikian0Wanlu Liu1Javier Gallego-Bartolomé2Steven E. Jacobsen3Department of Molecular, Cell, and Developmental Biology, University of CaliforniaDepartment of Molecular, Cell, and Developmental Biology, University of CaliforniaDepartment of Molecular, Cell, and Developmental Biology, University of CaliforniaDepartment of Molecular, Cell, and Developmental Biology, University of CaliforniaFew approaches for targeted manipulation of the epigenome are available in plants. Here, the authors adapt the dCas9-SunTag system to engineer targeted gene activation and site-specific manipulation of DNA methylation in Arabidopsis.https://doi.org/10.1038/s41467-019-08736-7
collection DOAJ
language English
format Article
sources DOAJ
author Ashot Papikian
Wanlu Liu
Javier Gallego-Bartolomé
Steven E. Jacobsen
spellingShingle Ashot Papikian
Wanlu Liu
Javier Gallego-Bartolomé
Steven E. Jacobsen
Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems
Nature Communications
author_facet Ashot Papikian
Wanlu Liu
Javier Gallego-Bartolomé
Steven E. Jacobsen
author_sort Ashot Papikian
title Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems
title_short Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems
title_full Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems
title_fullStr Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems
title_full_unstemmed Site-specific manipulation of Arabidopsis loci using CRISPR-Cas9 SunTag systems
title_sort site-specific manipulation of arabidopsis loci using crispr-cas9 suntag systems
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2019-02-01
description Few approaches for targeted manipulation of the epigenome are available in plants. Here, the authors adapt the dCas9-SunTag system to engineer targeted gene activation and site-specific manipulation of DNA methylation in Arabidopsis.
url https://doi.org/10.1038/s41467-019-08736-7
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AT javiergallegobartolome sitespecificmanipulationofarabidopsislociusingcrisprcas9suntagsystems
AT stevenejacobsen sitespecificmanipulationofarabidopsislociusingcrisprcas9suntagsystems
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