PolyPhenolic rich fraction of Terminalia paniculata attenuates obesity through inhibition of pancreatic amylase, lipase and 3T3-L1 adipocyte differentiation

PolyPhenolic rich fraction of Terminalia paniculata attenuates obesity through inhibition of pancreatic amylase, lipase and 3T3-L1 adipocyte differentiation

The prevalence of obesity and associated ailments have shown tremendous rise in recent times and drew the attention of researchers to explore effective therapeutic alternatives. In the present study, polyphenolic rich fraction of Terminalia paniculata bark (TPPF) was evaluated for its ability to inh...

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Main Authors: Muni Swamy Ganjayi, Balaji Meriga, Balaji Hari, Lokanatha Oruganti, Sreenivasulu Dasari, Ramgopal Mopuri
Format: Article
Language:English
Published: Elsevier 2017-12-01
Series:Journal of Nutrition & Intermediary Metabolism
Online Access:http://www.sciencedirect.com/science/article/pii/S2352385917302682
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spelling doaj-965e901eca9543e695d51e0c274811112020-11-24T22:28:18ZengElsevierJournal of Nutrition & Intermediary Metabolism2352-38592017-12-01101925PolyPhenolic rich fraction of Terminalia paniculata attenuates obesity through inhibition of pancreatic amylase, lipase and 3T3-L1 adipocyte differentiationMuni Swamy Ganjayi0Balaji Meriga1Balaji Hari2Lokanatha Oruganti3Sreenivasulu Dasari4Ramgopal Mopuri5Department of Biochemistry and Animal Physiology, Sri Venkateswara University, Tirupati, AP, 517502 IndiaDepartment of Biochemistry and Animal Physiology, Sri Venkateswara University, Tirupati, AP, 517502 India; Corresponding author.Department of Biochemistry and Animal Physiology, Sri Venkateswara University, Tirupati, AP, 517502 IndiaDepartment of Biochemistry and Animal Physiology, Sri Venkateswara University, Tirupati, AP, 517502 IndiaDepartment of Biochemistry and Animal Physiology, Sri Venkateswara University, Tirupati, AP, 517502 IndiaDepartment of Biotechnology, Sri Krishnadevaraya University, Anantapur, AP, IndiaThe prevalence of obesity and associated ailments have shown tremendous rise in recent times and drew the attention of researchers to explore effective therapeutic alternatives. In the present study, polyphenolic rich fraction of Terminalia paniculata bark (TPPF) was evaluated for its ability to inhibit adipogenesis, pancreatic lipase and amylase activity. The maximum inhibitory activity of lipase (78%) and amylase (81%) was noticed at 250 μg/mL of TPPF. During cytotoxicity assay with MTT, TPPF did not show any cytotoxic effect on 3T3-L1 cells up to a concentration of 400 μg/mL. Oil Red O staining of 3T3-L1 cells showed considerable reduction in adipocytes differentiation and lipid accumulation in the presence of TPPF (250 μg/mL) when compared with untreated 3T3-L1 cells. HPLC analysis of TPPF revealed the presence of Gallic acid, Ellagic acid and Quercetin as the major components. Our results suggest that, TPPF may be useful in attenuating obesity ailments. Keywords: Adipogenesis, α-Amylase, Pancreatic lipase, Terminalia paniculata and 3T3L1cellshttp://www.sciencedirect.com/science/article/pii/S2352385917302682
collection DOAJ
language English
format Article
sources DOAJ
author Muni Swamy Ganjayi
Balaji Meriga
Balaji Hari
Lokanatha Oruganti
Sreenivasulu Dasari
Ramgopal Mopuri
spellingShingle Muni Swamy Ganjayi
Balaji Meriga
Balaji Hari
Lokanatha Oruganti
Sreenivasulu Dasari
Ramgopal Mopuri
PolyPhenolic rich fraction of Terminalia paniculata attenuates obesity through inhibition of pancreatic amylase, lipase and 3T3-L1 adipocyte differentiation
Journal of Nutrition & Intermediary Metabolism
author_facet Muni Swamy Ganjayi
Balaji Meriga
Balaji Hari
Lokanatha Oruganti
Sreenivasulu Dasari
Ramgopal Mopuri
author_sort Muni Swamy Ganjayi
title PolyPhenolic rich fraction of Terminalia paniculata attenuates obesity through inhibition of pancreatic amylase, lipase and 3T3-L1 adipocyte differentiation
title_short PolyPhenolic rich fraction of Terminalia paniculata attenuates obesity through inhibition of pancreatic amylase, lipase and 3T3-L1 adipocyte differentiation
title_full PolyPhenolic rich fraction of Terminalia paniculata attenuates obesity through inhibition of pancreatic amylase, lipase and 3T3-L1 adipocyte differentiation
title_fullStr PolyPhenolic rich fraction of Terminalia paniculata attenuates obesity through inhibition of pancreatic amylase, lipase and 3T3-L1 adipocyte differentiation
title_full_unstemmed PolyPhenolic rich fraction of Terminalia paniculata attenuates obesity through inhibition of pancreatic amylase, lipase and 3T3-L1 adipocyte differentiation
title_sort polyphenolic rich fraction of terminalia paniculata attenuates obesity through inhibition of pancreatic amylase, lipase and 3t3-l1 adipocyte differentiation
publisher Elsevier
series Journal of Nutrition & Intermediary Metabolism
issn 2352-3859
publishDate 2017-12-01
description The prevalence of obesity and associated ailments have shown tremendous rise in recent times and drew the attention of researchers to explore effective therapeutic alternatives. In the present study, polyphenolic rich fraction of Terminalia paniculata bark (TPPF) was evaluated for its ability to inhibit adipogenesis, pancreatic lipase and amylase activity. The maximum inhibitory activity of lipase (78%) and amylase (81%) was noticed at 250 μg/mL of TPPF. During cytotoxicity assay with MTT, TPPF did not show any cytotoxic effect on 3T3-L1 cells up to a concentration of 400 μg/mL. Oil Red O staining of 3T3-L1 cells showed considerable reduction in adipocytes differentiation and lipid accumulation in the presence of TPPF (250 μg/mL) when compared with untreated 3T3-L1 cells. HPLC analysis of TPPF revealed the presence of Gallic acid, Ellagic acid and Quercetin as the major components. Our results suggest that, TPPF may be useful in attenuating obesity ailments. Keywords: Adipogenesis, α-Amylase, Pancreatic lipase, Terminalia paniculata and 3T3L1cells
url http://www.sciencedirect.com/science/article/pii/S2352385917302682
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