Establishing Human Male and Female Models of Cholinergic Neurons via Neurokine-Mediated Differentiation of LA-N-2 and LA-N-5 Neuroblastoma Cells

Establishing Human Male and Female Models of Cholinergic Neurons via Neurokine-Mediated Differentiation of LA-N-2 and LA-N-5 Neuroblastoma Cells

Summary: Cholinergic neurons control numerous primate-specific and sexually dimorphic brain functions. Here, we present our differentiation protocol for the closely related human female and male neuroblastoma-originated cell lines LA-N-2 and LA-N-5. Pro-cholinergic differentiation (with upregulation...

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Bibliographic Details
Main Authors: Sebastian Lobentanzer, Jochen Klein, Hermona Soreq
Format: Article
Language:English
Published: Elsevier 2020-12-01
Series:STAR Protocols
Subjects:
Cell culture
Model Organisms
Molecular Biology
Neuroscience
Cell Differentiation
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720301805

Internet

http://www.sciencedirect.com/science/article/pii/S2666166720301805

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