Isolation and Characterization of Novel EST-Derived Genic Markers in <i>Pisum sativum</i> (Fabaceae)

• Main Authors: Shalu Jain, Kevin E. McPhee
• Format: Article
• Language: English
• Published: Wiley 2013-10-01
• Series: Applications in Plant Sciences
• Subjects: comparative mapping; expressed sequence tags; lentil; marker-assisted selection; pea; synteny
• Online Access: http://www.bioone.org/doi/full/10.3732/apps.1300026

Premise of the study: Novel markers were developed for pea (<i>Pisum sativum</i>) from pea expressed sequence tags (ESTs) having significant homology to <i>Medicago truncatula</i> gene sequences to investigate genetic diversity, linkage mapping, and cross-species transferability. Methods and Results: Seventy-seven EST-derived genic markers were developed through comparative mapping between <i>M. truncatula</i> and <i>P. sativum</i> in which 75 markers produced PCR products and 33 were polymorphic among 16 pea genotypes. Conclusions: The novel markers described here will be useful for future genetic studies of <i>P. sativum</i>; their amplification in lentil (<i>Lens culinaris</i>) demonstrates their potential for use in closely related species.