Immunochemical Analysis of the Antimalarial Drugs Artemisinin and Artesunate

• Main Authors: Hiroyuki Tanaka, Madan K. Paudel, Ayako Takei, Junichi Sakoda, Thaweesak Juengwatanatrakul, Kaori Sasaki-Tabata, Waraporn Putalun, Wanchai De-Eknamkul, Oraphan Matangkasombut, Yukihiro Shoyama, Satoshi Morimoto
• Format: Article
• Language: English
• Published: MDPI AG 2012-11-01
• Series: Antibodies
• Subjects: artemisinin; artesunate; Artemisia annua; monoclonal antibody; recombinant Fab; ELISA
• Online Access: http://www.mdpi.com/2073-4468/1/3/273

We prepared a monoclonal antibody (mAb 1C1) showing specificity for artemisinin (AM) and artesunate (AS), and we developed an indirect competitive enzyme-linked immunosorbent assay (icELISA) using this novel mAb. Moreover, we prepared a recombinant antibody derived from mAb 1C1 in order to overcome insufficient mAb production by hybridoma culture. A recombinant antigen-binding fragment (Fab) was easily constructed using antibody manipulation technologies and was produced by microorganisms in high yield. We herein review immunochemical approaches for analysis of the antimalarial drugs AM and AS that were able to yield analysis results for multiple samples in a short period of time using simple and reliable protocols.