Green synthesis palladium nanoparticles mediated by white tea (Camellia sinensis) extract with antioxidant, antibacterial, and antiproliferative activities toward the human leukemia (MOLT-4) cell line

• Main Authors: Azizi S, Mahdavi Shahri M, Rahman HS, Rahim RA, Rasedee A, Mohamad R
• Format: Article
• Language: English
• Published: Dove Medical Press 2017-12-01
• Series: International Journal of Nanomedicine
• Subjects: green synthesis; Palladium nanoparticles; White tea; MOLT-4 cells; cytotoxicity; antibacterial activity
• Online Access: https://www.dovepress.com/green-synthesis-palladium-nanoparticles-mediated-by-white-tea-camellia-peer-reviewed-article-IJN

Susan Azizi,1 Mahnaz Mahdavi Shahri,2 Heshu Sulaiman Rahman,3–5 Raha Abdul Rahim,6 Abdullah Rasedee,5 Rosfarizan Mohamad1,7 1Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia; 2Department of Chemistry, Shiraz Branch, Islamic Azad University, Shiraz, Iran; 3College of Veterinary Medicine, University of Sulaimani, Sulaimani Nwe, 4College of Health Science, Komar University of Science and Technology (KUST), Chaq-Chaq Qularaise, Sulaimani City, Iraq; 5Faculty of Veterinary Medicine, 6Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, 7Laboratory of Biopolymer and Derivatives, Institute of Tropical Forestry and Forest Products, Universiti Putra Malaysia, UPM Serdang, Selangor, Malaysia Abstract: Among nanoparticles used for medical applications, palladium nanoparticles (PdNPs) are among the least investigated. This study was undertaken to develop PdNPs by green synthesis using white tea (W.tea; Camellia sinensis) extract to produce the [e-mail] NPs. The [e-mail] NPs were characterized by UV–vis spectroscopy and X-ray diffractometry, and evaluated with transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The [e-mail] NPs were spherical (size 6–18 nm) and contained phenols and flavonoids acquired from the W.tea extract. [e-mail] NPs has good 1-diphenyl-2-picrylhydrazyl (DPPH), OH, and NO-scavenging properties as well as antibacterial effects toward Staphylococcus epidermidis and Escherichia coli. MTT assay showed that [e-mail] NPs (IC50 =0.006 µM) were more antiproliferative toward the human leukemia (MOLT-4) cells than the W.tea extract (IC50 =0.894 µM), doxorubicin (IC50 =2.133 µM), or cisplatin (IC50 =0.013 µM), whereas they were relatively innocuous for normal human fibroblast (HDF-a) cells. The anticancer cell effects of [e-mail] NPs are mediated through the induction of apoptosis and G2/M cell-cycle arrest. Keywords: green synthesis, palladium nanoparticles, white tea, leukemic cells, cytotoxicity, medical application, nanobiotechnology