Comparison of polymorphism of STAT5A gene in Simmental and Holstein cows

• Main Authors: Mojtaba Najafi, GHodratallah Rahimi, Masoud Babaee, zarbakht Ansari
• Format: Article
• Language: fas
• Published: Shahid Bahonar University of Kerman 2010-11-01
• Series: مجله بیوتکنولوژی کشاورزی
• Subjects: polymorphism; pcr-sscp; stat5a; holstein and simmental cow
• Online Access: https://jab.uk.ac.ir/article_378_c5b6aeb943ec813bdd415401d0e2abfc.pdf
• ISSN: 2228-6705; 2228-6500

Signal transducer and activator of transcription 5A<em>(STAT5A)</em> is a crucial signaling protein mediating the biological effects of prolactin hormone which in turn activates the transcription of milk proteins genes. In order to study the polymorphisms of <em>STAT5A</em> gene total blood samples were collected randomly from one hundred of Holstein and Simmental cattle. DNA was extracted using modified salting out method and polymerase chain reactions (PCR) were carried out using a specific primer pairs for amplification a fragment of 281 bp from part of exon 15 and intron 16 of <em>STAT5A</em> gene. All of individuals were genotyped by single stranded conformation polymorphism (SSCP) analyses using silver staining.  Two SSCP patterns of A and B were identified with the frequency of 0.91 and 0.09 and 0.88 and 0.12 in Holstein and Simmental cattle, respectively. Two genotypes of AA (0.82) and AB (0.18) were found in Holstein and AA (0.76) and AB (0.24) in Simmental individuals. The results revealed that the marker site of STAT5A gene is polymorph in studied Holstein and Simmental cattle and the SSCP technique has a great potential for detection of such polymorphism. Furthermore, a comparison of gene and genotype frequencies showed no any statistical differences (P>0.05) between Holstein and Simmental cattle breed that have genotyped in the present study.