Melatonin Inhibits Apoptosis and Oxidative Stress of Mouse Leydig Cells via a SIRT1-Dependent Mechanism

• Main Authors: Gaoqing Xu, Jing Zhao, Hongyu Liu, Jun Wang, Wenfa Lu
• Format: Article
• Language: English
• Published: MDPI AG 2019-08-01
• Series: Molecules
• Subjects: melatonin; apoptosis; oxidative stress; SIRT1; Leydig cells; mouse
• Online Access: https://www.mdpi.com/1420-3049/24/17/3084

The purpose of the present study is to examine the effects of melatonin on apoptosis and oxidative stress in mouse Leydig cells and to elucidate the mechanisms responsible for these effects. Our results indicated that 10 ng/mL of melatonin significantly promoted cell viability, the ratio of EdU-positive (5-Ethynyl-2&#8242;-deoxyuridine) cells, and increased the mRNA expression of proliferating cell nuclear antigen (<i>PCNA</i>), cyclin D1(<i>CCND1</i>), and cell division control protein 42 (<i>CDC42</i>) (<i>p</i> &lt; 0.05). We also observed that melatonin inhibited apoptosis of mouse Leydig cells, accompanied with increased B-cell lymphoma-2 (<i>BCL-2</i>) and decreased BCL2 associated X (<i>BAX</i>) mRNA and protein expression. Moreover, addition of melatonin significantly decreased the reactive oxygen species (ROS) production and malondialdehyde (MDA) and 8-hydroxy-2&#8242;-deoxyguanosine (8-OHdG) levels, while it increased superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels (<i>p</i> &lt; 0.05). In addition, we also found that melatonin increased the expression of <i>SIRT1</i> (Silent information regulator 1) (<i>p</i> &lt; 0.05). To explore the role of SIRT1 signaling in melatonin-induced cells, mouse Leydig cells were pretreated with EX527, an inhibitor of SIRT1. The protective effects of melatonin on mouse Leydig cells were reversed by EX527, as shown by decreased cell proliferation and increased cell apoptosis and oxidative stress. In summary, our results demonstrated that melatonin inhibited apoptosis and oxidative stress of mouse Leydig cells through a SIRT1-dependent mechanism.