Monitoring Schizosaccharomyces pombe genome stress by visualizing end-binding protein Ku

Studies of genome stability have exploited visualization of fluorescently tagged proteins in live cells to characterize DNA damage, checkpoint, and repair responses. In this report, we describe a new tool for fission yeast, a tagged version of the end-binding protein Pku70 which is part of the KU pr...

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Main Authors:	Chance E. Jones, Susan L. Forsburg
Format:	Article
Language:	English
Published:	The Company of Biologists 2021-02-01
Series:	Biology Open
Subjects:	dna damage ku fission yeast genome stability microscopy
Online Access:	http://bio.biologists.org/content/10/2/bio054346

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spelling	doaj-94e36ee0e41c47a39de4eecb8721c4ab2021-06-02T14:56:34ZengThe Company of BiologistsBiology Open2046-63902021-02-0110210.1242/bio.054346054346Monitoring Schizosaccharomyces pombe genome stress by visualizing end-binding protein KuChance E. Jones0Susan L. Forsburg1 Program in Molecular & Computational Biology, University of Southern California, Los Angeles, CA 90089, USA Program in Molecular & Computational Biology, University of Southern California, Los Angeles, CA 90089, USA Studies of genome stability have exploited visualization of fluorescently tagged proteins in live cells to characterize DNA damage, checkpoint, and repair responses. In this report, we describe a new tool for fission yeast, a tagged version of the end-binding protein Pku70 which is part of the KU protein complex. We compare Pku70 localization to other markers upon treatment to various genotoxins, and identify a unique pattern of distribution. Pku70 provides a new tool to define and characterize DNA lesions and the repair response.http://bio.biologists.org/content/10/2/bio054346dna damagekufission yeastgenome stabilitymicroscopy
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Chance E. Jones Susan L. Forsburg
spellingShingle	Chance E. Jones Susan L. Forsburg Monitoring Schizosaccharomyces pombe genome stress by visualizing end-binding protein Ku Biology Open dna damage ku fission yeast genome stability microscopy
author_facet	Chance E. Jones Susan L. Forsburg
author_sort	Chance E. Jones
title	Monitoring Schizosaccharomyces pombe genome stress by visualizing end-binding protein Ku
title_short	Monitoring Schizosaccharomyces pombe genome stress by visualizing end-binding protein Ku
title_full	Monitoring Schizosaccharomyces pombe genome stress by visualizing end-binding protein Ku
title_fullStr	Monitoring Schizosaccharomyces pombe genome stress by visualizing end-binding protein Ku
title_full_unstemmed	Monitoring Schizosaccharomyces pombe genome stress by visualizing end-binding protein Ku
title_sort	monitoring schizosaccharomyces pombe genome stress by visualizing end-binding protein ku
publisher	The Company of Biologists
series	Biology Open
issn	2046-6390
publishDate	2021-02-01
description	Studies of genome stability have exploited visualization of fluorescently tagged proteins in live cells to characterize DNA damage, checkpoint, and repair responses. In this report, we describe a new tool for fission yeast, a tagged version of the end-binding protein Pku70 which is part of the KU protein complex. We compare Pku70 localization to other markers upon treatment to various genotoxins, and identify a unique pattern of distribution. Pku70 provides a new tool to define and characterize DNA lesions and the repair response.
topic	dna damage ku fission yeast genome stability microscopy
url	http://bio.biologists.org/content/10/2/bio054346
work_keys_str_mv	AT chanceejones monitoringschizosaccharomycespombegenomestressbyvisualizingendbindingproteinku AT susanlforsburg monitoringschizosaccharomycespombegenomestressbyvisualizingendbindingproteinku
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Monitoring Schizosaccharomyces pombe genome stress by visualizing end-binding protein Ku

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