Monitoring Schizosaccharomyces pombe genome stress by visualizing end-binding protein Ku

Monitoring Schizosaccharomyces pombe genome stress by visualizing end-binding protein Ku

Studies of genome stability have exploited visualization of fluorescently tagged proteins in live cells to characterize DNA damage, checkpoint, and repair responses. In this report, we describe a new tool for fission yeast, a tagged version of the end-binding protein Pku70 which is part of the KU pr...

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Bibliographic Details
Main Authors: Chance E. Jones, Susan L. Forsburg
Format: Article
Language:English
Published: The Company of Biologists 2021-02-01
Series:Biology Open
Subjects:
dna damage
ku
fission yeast
genome stability
microscopy
Online Access:http://bio.biologists.org/content/10/2/bio054346
Description
Summary:Studies of genome stability have exploited visualization of fluorescently tagged proteins in live cells to characterize DNA damage, checkpoint, and repair responses. In this report, we describe a new tool for fission yeast, a tagged version of the end-binding protein Pku70 which is part of the KU protein complex. We compare Pku70 localization to other markers upon treatment to various genotoxins, and identify a unique pattern of distribution. Pku70 provides a new tool to define and characterize DNA lesions and the repair response.
ISSN:2046-6390

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