Full-length infectious clone of a low passage dengue virus serotype 2 from Brazil

• Main Authors: Jefferson José da Silva Santos, Tereza Magalhães, José Valter Joaquim Silva Junior, Andréa Nazaré Monteiro Rangel da Silva, Marli Tenório Cordeiro, Laura Helena Vega Gonzales Gil
• Format: Article
• Language: English
• Published: Instituto Oswaldo Cruz, Ministério da Saúde 2015-01-01
• Series: Memórias do Instituto Oswaldo Cruz.
• Subjects: infectious clone; yeast homologous recombination; flavivirus
• Online Access: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762015005050053&lng=en&tlng=en
• ISSN: 1678-8060

Full-length dengue virus (DENV) cDNA clones are an invaluable tool for many studies, including those on the development of attenuated or chimeric vaccines and on host-virus interactions. Furthermore, the importance of low passage DENV infectious clones should be highlighted, as these may harbour critical and unique strain-specific viral components from field-circulating isolates. The successful construction of a functional Brazilian low passage DENV serotype 2 full-length clone through homologous recombination reported here supports the use of a strategy that has been shown to be highly useful by our group for the development of flavivirus infectious clones and replicons.