Diagnostics of Severe Fever with Thrombocytopenia Syndrome – Disease, Caused by Novel Phlebovirus

• Main Authors: T. E. Sizikova, V. N. Lebedev, N. V. Boyarskaya, S. V. Borisevich
• Format: Article
• Language: Russian
• Published: Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” 2016-09-01
• Series: Проблемы особо опасных инфекций
• Subjects: severe fever with thrombocytopenia syndrome; severe fever with thrombocytopenia syndrome virus, phlebovirus, genomic rna, primer, reverse transcription-polymerase chain reaction, fluorescent antibodies method; enzyme linked immunosorbent assay; neutralization testing; sensitivity; specificity
• Online Access: https://journal.microbe.ru/jour/article/view/320

In 2009, a novel virus, named severe fever with thrombocytopenia syndrome (SFTS) virus, was isolated from a patient in China. The illness caused by this novel virus is characterized by a sudden onset of fever and respiratory or gastrointestinal disorders, followed by progressive thrombocytopenia and leucocytopenia, the case-fatality rate amounting to 6–30 %. Genomic sequencing of the isolated agent indicated that the SFTS virus constituted a new (third) group of Phlebovirus genus, Bunyaviridae family. Presently, different means for specific diagnostics of SFTS (real-time reverse transcription polymerase chain reaction, indirect fluorescent antibodies method, enzyme-linked immune-sorbent assay) are developed. Constructing of diagnostic kits, basic characteristics of methods for determination of causative agent of infection or specific antibodies against it are considered in this review.