Loss of Suppressor of Fused in Mid-Corticogenesis Leads to the Expansion of Intermediate Progenitors

Neural progenitors in the embryonic neocortex must be tightly regulated in order to generate the correct number and projection neuron subtypes necessary for the formation of functional neocortical circuits. In this study, we show that the intracellular protein Suppressor of Fused (Sufu) regulates th...

Full description

Bibliographic Details
Main Authors:	Odessa R. Yabut, Hui Xuan Ng, Gloria Fernandez, Keejung Yoon, Jeremy Kuhn, Samuel J. Pleasure
Format:	Article
Language:	English
Published:	MDPI AG 2016-09-01
Series:	Journal of Developmental Biology
Subjects:	Suppressor of Fused Sonic Hedgehog Gli3 corticogenesis neurogenesis
Online Access:	http://www.mdpi.com/2221-3759/4/4/29

id	doaj-94660155f7bd4b32886bbe2f57277bf3
record_format	Article
spelling	doaj-94660155f7bd4b32886bbe2f57277bf32020-11-24T22:25:49ZengMDPI AGJournal of Developmental Biology2221-37592016-09-01442910.3390/jdb4040029jdb4040029Loss of Suppressor of Fused in Mid-Corticogenesis Leads to the Expansion of Intermediate ProgenitorsOdessa R. Yabut0Hui Xuan Ng1Gloria Fernandez2Keejung Yoon3Jeremy Kuhn4Samuel J. Pleasure5Department of Neurology, University of California San Francisco, San Francisco, CA 94158, USADepartment of Neurology, University of California San Francisco, San Francisco, CA 94158, USADepartment of Neurology, University of California San Francisco, San Francisco, CA 94158, USADepartment of Neurology, University of California San Francisco, San Francisco, CA 94158, USADepartment of Neurology, University of California San Francisco, San Francisco, CA 94158, USADepartment of Neurology, University of California San Francisco, San Francisco, CA 94158, USANeural progenitors in the embryonic neocortex must be tightly regulated in order to generate the correct number and projection neuron subtypes necessary for the formation of functional neocortical circuits. In this study, we show that the intracellular protein Suppressor of Fused (Sufu) regulates the proliferation of intermediate progenitor (IP) cells at later stages of corticogenesis to affect the number of Cux1+ upper layer neurons in the postnatal neocortex. This correlates with abnormal levels of the repressor form of Gli3 (Gli3R) and the ectopic expression of Patched 1 (Ptch1), a Sonic Hedgehog (Shh) target gene. These studies reveal that the canonical role of Sufu as an inhibitor of Shh signaling is conserved at later stages of corticogenesis and that Sufu plays a crucial role in regulating neuronal number by controlling the cell cycle dynamics of IP cells in the embryonic neocortex.http://www.mdpi.com/2221-3759/4/4/29Suppressor of FusedSonic HedgehogGli3corticogenesisneurogenesis
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Odessa R. Yabut Hui Xuan Ng Gloria Fernandez Keejung Yoon Jeremy Kuhn Samuel J. Pleasure
spellingShingle	Odessa R. Yabut Hui Xuan Ng Gloria Fernandez Keejung Yoon Jeremy Kuhn Samuel J. Pleasure Loss of Suppressor of Fused in Mid-Corticogenesis Leads to the Expansion of Intermediate Progenitors Journal of Developmental Biology Suppressor of Fused Sonic Hedgehog Gli3 corticogenesis neurogenesis
author_facet	Odessa R. Yabut Hui Xuan Ng Gloria Fernandez Keejung Yoon Jeremy Kuhn Samuel J. Pleasure
author_sort	Odessa R. Yabut
title	Loss of Suppressor of Fused in Mid-Corticogenesis Leads to the Expansion of Intermediate Progenitors
title_short	Loss of Suppressor of Fused in Mid-Corticogenesis Leads to the Expansion of Intermediate Progenitors
title_full	Loss of Suppressor of Fused in Mid-Corticogenesis Leads to the Expansion of Intermediate Progenitors
title_fullStr	Loss of Suppressor of Fused in Mid-Corticogenesis Leads to the Expansion of Intermediate Progenitors
title_full_unstemmed	Loss of Suppressor of Fused in Mid-Corticogenesis Leads to the Expansion of Intermediate Progenitors
title_sort	loss of suppressor of fused in mid-corticogenesis leads to the expansion of intermediate progenitors
publisher	MDPI AG
series	Journal of Developmental Biology
issn	2221-3759
publishDate	2016-09-01
description	Neural progenitors in the embryonic neocortex must be tightly regulated in order to generate the correct number and projection neuron subtypes necessary for the formation of functional neocortical circuits. In this study, we show that the intracellular protein Suppressor of Fused (Sufu) regulates the proliferation of intermediate progenitor (IP) cells at later stages of corticogenesis to affect the number of Cux1+ upper layer neurons in the postnatal neocortex. This correlates with abnormal levels of the repressor form of Gli3 (Gli3R) and the ectopic expression of Patched 1 (Ptch1), a Sonic Hedgehog (Shh) target gene. These studies reveal that the canonical role of Sufu as an inhibitor of Shh signaling is conserved at later stages of corticogenesis and that Sufu plays a crucial role in regulating neuronal number by controlling the cell cycle dynamics of IP cells in the embryonic neocortex.
topic	Suppressor of Fused Sonic Hedgehog Gli3 corticogenesis neurogenesis
url	http://www.mdpi.com/2221-3759/4/4/29
work_keys_str_mv	AT odessaryabut lossofsuppressoroffusedinmidcorticogenesisleadstotheexpansionofintermediateprogenitors AT huixuanng lossofsuppressoroffusedinmidcorticogenesisleadstotheexpansionofintermediateprogenitors AT gloriafernandez lossofsuppressoroffusedinmidcorticogenesisleadstotheexpansionofintermediateprogenitors AT keejungyoon lossofsuppressoroffusedinmidcorticogenesisleadstotheexpansionofintermediateprogenitors AT jeremykuhn lossofsuppressoroffusedinmidcorticogenesisleadstotheexpansionofintermediateprogenitors AT samueljpleasure lossofsuppressoroffusedinmidcorticogenesisleadstotheexpansionofintermediateprogenitors
_version_	1725756129219182592

Loss of Suppressor of Fused in Mid-Corticogenesis Leads to the Expansion of Intermediate Progenitors

Similar Items