FUNCTIONAL ACTIVITY OF IFNα- AND IL-4-INDUCED HUMAN DENDRITIC CELLS: A COMPARATIVE STUDY

• Main Authors: O. Yu. Leplina, M. A. Tikhonova, T. V. Tyrinova, E. A. Alyamkina, S. S. Bogachev, A. A, Ostanin, E. R. Chernykh
• Format: Article
• Language: Russian
• Published: SPb RAACI 2014-07-01
• Series: Medicinskaâ Immunologiâ
• Subjects: dendritic cells; allostimulatory activity; th1-/th2-сytokines; regulatory t-cells
• Online Access: https://www.mimmun.ru/mimmun/article/view/22

We have performed a comparative analysis of allostimulatory, Th1/Th2-stimulatory andtolerogenic activities of dendritic cells (DCs) derived from healthy donors (n = 52). The DCs were generatedin vitro with IFN-alpha (IFN-DCs) or IL-4 (IL-4-DCs). It was shown that IL-4-DCs and IFN-DCs didnot differ in their key functional characteristics, i.e., in their ability to stimulate proliferation of T-cells inresponse to alloantigens, and to induce generation of T-regulatory cells in mixed leukocyte culture. IFN- andIL-4-induced DCs possess similar ability of boosting T cells to produce Th1/proinflammatory (IFNγ, IL-2,IL-1β, TNFα, IL-12, IL-17) and Th2/antiinflammatory cytokines (IL-4, IL-6, IL-10, IL-13), growth factors(G-CSF, GM-CSF, IL-7) and chemokines (IL-8, MIP-1β). Nevertheless, IFN-DCs have more pronouncedstimulatory effect upon Th1 and Th2 cells, thus manifesting as a significantly higher IFNγ, IL-5 and MIP-1βproduction. IFN-DCs were characterized by more prominent ability to activate Th1-cells, and by moderateTh2-stimulatory activity, which is absent in IL-4-DCs. These data strongly suggest that IFN-DCs may presenta quite promising cellular tool for development of therapeutic vaccines aiming to induce/enhance the immuneresponse.